Anti-Oct-2 抗体 [EP543Y] (ab33919)
![Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)](https://www.abcam.co.jp/ps/products/33/ab33919/Images/ab33919-553682-anti-oct-2-antibody-ep543Y-western-blot-human-raji-daudi.jpg "Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP543Y] to Oct-2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Oct-2 antibody [EP543Y]
Oct-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP543Y] to Oct-2 -
由来種
Rabbit -
特異性
This antibody may detect the isoform 2.
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アプリケーション
適用あり: WBmore details
適用なし: ChIP or Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Raji and Daudi whole cell lysates.
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特記事項
This product has switched from a hybridoma to recombinant production method on 13th September 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP543Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33919の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 51 kDa.
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| 特記事項 |
|---|
|
WB
1/10000. Predicted molecular weight: 51 kDa. |
ターゲット情報
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機能
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
組織特異性
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
配列類似性
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5452 Human
- Omim: 164176 Human
- SwissProt: P09086 Human
- Unigene: 147381 Human
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別名
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
画像
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Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919)All lanes : Anti-Oct-2 antibody [EP543Y] (ab33919) at 1/10000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab33919 は 3 報の論文で使用されています。
- Wei L et al. Functional role of PLCE1 intronic insertion variant associated with susceptibility to esophageal squamous cell carcinoma. Carcinogenesis 39:191-201 (2018). PubMed: 29106514
- Oguri T et al. Organic cation transporter 6 directly confers resistance to anticancer platinum drugs. Biomed Rep 5:639-643 (2016). WB ; Human . PubMed: 27882231
- Winiarska M et al. Prenyltransferases Regulate CD20 Protein Levels and Influence Anti-CD20 Monoclonal Antibody-mediated Activation of Complement-dependent Cytotoxicity. J Biol Chem 287:31983-93 (2012). ChIP . PubMed: 22843692