AB33919

Anti-Oct-2 抗体 [EP543Y]

Anti-Oct-2 antibody [EP543Y]

RabMAb
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(3 Publications)

Rabbit Monoclonal Oct-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

OCT2, OTF2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding protein 2, Octamer-binding transcription factor 2, Oct-2, OTF-2

1 Images

Lab

Western blot - Anti-Oct-2 antibody [EP543Y] (AB33919)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Oct-2 antibody [EP543Y] (ab33919) at 1/10000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP543Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody may detect the isoform 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" } } }

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製品の詳細

This product has switched from a hybridoma to recombinant production method on 13th September 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Oct-2 also known as 9A2 or POU2F2 is a transcription factor with a molecular mass of approximately 50-55 kDa. It belongs to the POU family of transcription factors. Oct-2 is expressed widely in various tissues but shows higher expression levels in B lymphocytes and the central nervous system. This protein binds to the octamer DNA sequence motif which regulates the activity of several genes involved in immune function and neural development. Its expression pattern and DNA binding properties make Oct-2 essential in controlling transcriptional activation and repression.

Biological function summary

Oct-2 regulates the expression of target genes by binding to octamer motifs within promoters and enhancers. In the immune system Oct-2 plays a significant role in B cell differentiation and maturation. It acts as an essential transcriptional regulator of immunoglobulin gene expression. In neural tissues Oct-2 influences genes involved in neural differentiation and development. Oct-2 often does not act alone; it forms part of multi-protein complexes interacting with coactivators and corepressors to modulate gene expression tightly.

Pathways

Oct-2 participates in critical transcriptional regulatory networks. It is important in the immune response pathway controlling genes involved in B cell activation and antibody production. Oct-2 also interacts with Oct-1 another member of the POU family in controlling gene expression patterns related to adaptive immunity. Additionally Oct-2 influences pathways involved in neural development working alongside transcription factors like Pax-5 to regulate genes critical for the growth and differentiation of neuronal cells.

Oct-2 has been implicated in certain B cell-related malignancies and neurological disorders. Abnormal expression or mutation of Oct-2 can contribute to the development of lymphoma by altering the normal regulation of proliferation-related genes in B cells. Oct-2 also has connections to neurodevelopmental disorders where dysregulation of neural-specific genes controlled by Oct-2 may contribute to disease pathology. Understanding Oct-2's interaction with related proteins such as EBF1 in these disorders provides insights into the complex genetic regulation involved in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed : 2904654, PubMed : 7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed : 2901913, PubMed : 2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed : 10480874).. Isoform 5. Activates the U2 small nuclear RNA (snRNA) promoter.

See full target information POU2F2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 39:191-201 PubMed29106514

2017

Functional role of PLCE1 intronic insertion variant associated with susceptibility to esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Wei,Mingming Shao,Yanjie Zhao,Jian Zheng,Jiahui Chu,Jiang Chang,Xinxin Cheng,Qionghua Cui,Linna Peng,Yingying Luo,Wenle Tan,Wen Tan,Dongxin Lin,Chen Wu

Biomedical reports 5:639-643 PubMed27882231

2016

Organic cation transporter 6 directly confers resistance to anticancer platinum drugs.

Applications

Species

Human

Tetsuya Oguri,Eiji Kunii,Satoshi Fukuda,Kazuki Sone,Takehiro Uemura,Osamu Takakuwa,Yoshihiro Kanemitsu,Hirotsugu Ohkubo,Masaya Takemura,Ken Maeno,Yutaka Ito,Akio Niimi

The Journal of biological chemistry 287:31983-93 PubMed22843692

2012

Prenyltransferases regulate CD20 protein levels and influence anti-CD20 monoclonal antibody-mediated activation of complement-dependent cytotoxicity.

Applications

ChIP

Species

Unspecified reactive species

Magdalena Winiarska,Dominika Nowis,Jacek Bil,Eliza Glodkowska-Mrowka,Angelika Muchowicz,Malgorzata Wanczyk,Kamil Bojarczuk,Michal Dwojak,Malgorzata Firczuk,Ewa Wilczek,Malgorzata Wachowska,Katarzyna Roszczenko,Marta Miaczynska,Justyna Chlebowska,Grzegorz Wladyslaw Basak,Jakub Golab

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct-2 抗体 [EP543Y]

Anti-Oct-2 antibody [EP543Y]

Western blot - Anti-Oct-2 antibody [EP543Y] (AB33919)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

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Abcam product promise