Anti-Ochratoxin A 抗体 [3C5] (ab23965)

The answer is Yes, the antibody ab23965 will recognize Ochratoxin A when it would be coupled to HRP.

The antibody ab23965 has been tested on Ochratoxin A from Sigma, Ref. O 1877. http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/O1877 The following protocol was used: Ochratoxin A is coupled on BSA and used as antigen. -This antigen is coated in a 96 wells plate (EIA/RIA high binding Ref. Corning Costar 3590) at ~ 10µg/ml in bicabonate buffer pH 9.5. Incubation 1 hour at 37°C with shaking. - Saturation : coupling solution is eliminated by turning over the plate. Add 150 µl of BSA 0.5% in PBS. Incubation 1/2 hour at 37°C with shaking. - Eliminate the BSA solution. Add 0.1ml of ab23965 diluted at 1/5000 in PBS/Tween 20(0.1 %). Incubation 1/2 hour to 1 hour at 37°C with shaking. - Wash the plate 4 time with PBS/Tween 20 (0.1%) or TBS/Tween 20 (0.1%). Add 0.1ml of diluted secondary antibody. Incubation 1/2 hour at 37°C with shaking. - Wash the plate 4 time with PBS/Tween 20 (0.1%) or TBS/Tween 20 (0.1%). Add 0.1ml Chromogen. (to be chosen according the enzyme used for labelling the secondary antibody). -Stop the reaction. -Read the OD of the wells. I will add this information to the datasheet and hope it will be useful to you,

Thank you for your enquiry. This antibody has not been epitope-mapped, so the epitope could reside anywhere along the length of the protein. Therefore, it is impossible to say whether the epitope of this antibody overlaps with the carboxylic group. I am sorry I couldn't be more helpful. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, the coupling to BSA method is confidential and cannot be released. I am sorry I can't be more helpful in this occasion. Should you require anything else, please do not hesitate to contact me.