Anti-Occludin 抗体 (ab31721)
Key features and details
- Rabbit polyclonal to Occludin
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Occludin antibody
Occludin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Occludin -
由来種
Rabbit -
特異性
New batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab216327 as an alternative for ICC/IF and IHC-P. The immunogen used to raise this antibody has 78% homology with the Mouse Occludin protein and the Rat Occludin protein. Some customers have successfully used ab31721 with Mouse/Rat lysates/tissue, however we have not been successful detecting Occludin in these species in our own testing. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Dog, Pig
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Occludin.
Immunogen の所有権に関して (Peptide available as ab34440.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31721の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
1/250. Detects a band of approximately 62 kDa (predicted molecular weight: 59 kDa).
|
| 特記事項 |
|---|
|
WB
1/250. Detects a band of approximately 62 kDa (predicted molecular weight: 59 kDa). |
ターゲット情報
-
機能
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. -
組織特異性
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. -
関連疾患
Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay. -
配列類似性
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. -
ドメイン
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. -
細胞内局在
Membrane. Cell junction > tight junction. - Information by UniProt
-
参照データベース
- Entrez Gene: 403844 Dog
- Entrez Gene: 100506658 Human
- Entrez Gene: 397236 Pig
- Omim: 602876 Human
- SwissProt: Q28269 Dog
- SwissProt: Q16625 Human
- Unigene: 592605 Human
-
別名
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
see all
画像
-
Western blot - Anti-Occludin antibody (ab31721)All lanes : Anti-Occludin antibody (ab31721) at 1/250 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate (Low Occludin expression) at 20 µg
Lane 4 : HepG2 whole cell lysate (High Occludin expression) at 20 µg
Predicted band size: 59 kDaLanes 1 - 4: Merged signal (red and green). Green - ab31721 observed at 59 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab31721 was shown to recognize Occludin in wild-type HAP1 cells as signal was lost at the expected MW in OCLN (Occludin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and OCLN (Occludin) knockout samples were subjected to SDS-PAGE. Ab31721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Occludin antibody (ab31721)Lane 1 : Anti-Occludin antibody [EPR8208] (ab167161) at 1/5000 dilution
Lane 2 : Anti-Occludin antibody (ab31721) at 1/1000 dilution
Lane 3 : Anti-Occludin antibody [EPR20992] (ab216327) at 1/5000 dilution
All lanes :Recombinant Human Occludin protein (ab114189)
Lysates/proteins at 0.025 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 5.5 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (185)
ab31721 は 185 報の論文で使用されています。
- Qiu T & Tucker AS Mechanisms driving vestibular lamina formation and opening in the mouse. J Anat 242:224-234 (2023). PubMed: 36181694
- Jeon JH et al. Tanshinone IIA-loaded nanoparticles and neural stem cell combination therapy improves gut homeostasis and recovery in a pig ischemic stroke model. Sci Rep 13:2520 (2023). PubMed: 36781906
- Fu R et al. Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets. J Anim Sci Biotechnol 14:44 (2023). PubMed: 36932457
- Qiu Q et al. P53 Deficiency Accelerate Esophageal Epithelium Intestinal Metaplasia Malignancy. Biomedicines 11:N/A (2023). PubMed: 36979860
- Chen K et al. Changes in gut microbiota linked to a prevention of cardiac remodeling induced by hypertension in spontaneously hypertensive rats fed a pawpaw fruit diet. Heliyon 9:e15576 (2023). PubMed: 37131439