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AB31721

Anti-Occludin 抗体

Anti-Occludin antibody

4

(19 Reviews)

|

(185 Publications)

Rabbit Polyclonal Occludin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 185 publications.

別名を表示する

Occludin, OCLN

1 Images
Western blot - Anti-Occludin antibody (AB31721)
  • WB

Lab

Western blot - Anti-Occludin antibody (AB31721)

Lanes 1 - 4 : Merged signal (red and green). Green - ab31721 observed at 59 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab31721 was shown to recognize Occludin in wild-type HAP1 cells as signal was lost at the expected MW in OCLN (Occludin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and OCLN (Occludin) knockout samples were subjected to SDS-PAGE. ab31721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Occludin antibody (ab31721) at 1/250 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 40 µg

Lane 2:

OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg

Lane 3:

HeLa whole cell lysate (Low Occludin expression) at 20 µg

Lane 4:

HepG2 whole cell lysate (High Occludin expression) at 20 µg

Predicted band size: 59 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

New batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab216327 as an alternative for ICC/IF and IHC-P. The immunogen used to raise this antibody has 78% homology with the Mouse Occludin protein and the Rat Occludin protein. Some customers have successfully used ab31721 with Mouse/Rat lysates/tissue, however we have not been successful detecting Occludin in these species in our own testing.

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Reactivity data

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下記製品もご検討ください

AB216327

Anti-Occludin antibody [EPR20992]

4
7 Reviews
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Occludin is a protein that plays an important mechanical role in forming tight junctions which are specialized connections between neighboring cell membranes. Alternative names for this target include occludin-1. The occludin protein has a molecular weight of approximately 65 kDa. It is expressed in a variety of tissues but levels tend to be high in epithelial and endothelial cells where tight junctions are essential for barrier functions. Occludin acts as integral membrane protein contributing to the complex structures that seal spaces between cells.
Biological function summary

Occludin serves as an important component in maintaining cell-to-cell adhesion within tight junction complexes. It interacts with other tight junction proteins such as claudins and junctional adhesion molecules to regulate paracellular permeability. These interactions help maintain selective barrier properties of epithelial and endothelial layers preventing the passage of large molecules and pathogens while allowing passage of ions and small molecules. Occludin stabilizes the tight junctions by interacting with the cytoskeleton and signaling proteins that are important for tight junction assembly and maintenance.

Pathways

Occludin functions prominently in the regulation of tight junction integrity and barrier function. It participates in pathways such as the tight junction signaling pathway and the paracellular transport pathway. Occludin interacts with ZO-1 a scaffolding protein bridging its interaction with the actin cytoskeleton which supports the structural organization and function of tight junctions. This connectivity allows for regulation of the permeability and transport across the epithelial barrier.

Disruptions in occludin function are connected to conditions such as inflammatory bowel disease (IBD) and some cancers. In IBD abnormal occludin expression can compromise intestinal barrier function leading to increased intestinal permeability and an inflammatory response. In certain cancers loss of occludin expression is linked to increased invasiveness and metastasis due to weakened cell adhesion. The dysfunction of proteins like ZO-1 which interact with occludin in these pathways also contributes to these pathological states highlighting the importance of occludin's regulatory roles in maintaining cellular barrier integrity.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
See full target information OCLN
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文献 (185)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:13669-13679 PubMed38048213

2023

Atorvastatin mitigates memory deficits and brain monocyte infiltration in chronic hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fengchao Gong,Qian Shi,Xiaojie Mou,Kang Wang,Qianqian Wang,Haitao Wang

Animals : an open access journal from MDPI 13: PubMed38003115

2023

Dietary Supplementation with Lysozyme-Cinnamaldehyde Conjugates Enhances Feed Conversion Efficiency by Improving Intestinal Health and Modulating the Gut Microbiota in Weaned Piglets Infected with Enterotoxigenic .

Applications

Unspecified application

Species

Unspecified reactive species

Zhezhe Tian,Jiaming Chen,Tongbin Lin,Junhua Zhu,Haoyang Gan,Fang Chen,Shihai Zhang,Wutai Guan

Alzheimer's research & therapy 15:153 PubMed37700368

2023

Is later-life depression a risk factor for Alzheimer's disease or a prodromal symptom: a study using post-mortem human brain tissue?

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey I Sinclair,Asher Mohr,Mizuki Morisaki,Martin Edmondson,Selina Chan,A Bone-Connaughton,Gustavo Turecki,Seth Love

Heliyon 9:e15576 PubMed37131439

2023

Changes in gut microbiota linked to a prevention of cardiac remodeling induced by hypertension in spontaneously hypertensive rats fed a pawpaw fruit diet.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Shaoyu Wu,Yiqing Guan,Yunci Ma,Yu Huang,Xin Liu,Dongling Quan,Jingru Zhang,Lin Lv,Guohua Zhang

Journal of animal science and biotechnology 14:44 PubMed36932457

2023

Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Runqi Fu,Chan Liang,Daiwen Chen,Gang Tian,Ping Zheng,Jun He,Jie Yu,Xiangbing Mao,Yuheng Luo,Junqiu Luo,Bing Yu

Biomedicines 11: PubMed36979860

2023

P53 Deficiency Accelerate Esophageal Epithelium Intestinal Metaplasia Malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Quanpeng Qiu,Gang Guo,Xiaolong Guo,Xiake Hu,Tianyu Yu,Gaixia Liu,Haowei Zhang,Yinnan Chen,Junjun She

Scientific reports 13:2520 PubMed36781906

2023

Tanshinone IIA-loaded nanoparticles and neural stem cell combination therapy improves gut homeostasis and recovery in a pig ischemic stroke model.

Applications

Unspecified application

Species

Unspecified reactive species

Julie H Jeon,Erin E Kaiser,Elizabeth S Waters,Xueyuan Yang,Jeferson M Lourenco,Madison M Fagan,Kelly M Scheulin,Sydney E Sneed,Soo K Shin,Holly A Kinder,Anil Kumar,Simon R Platt,Jeongyoun Ahn,Kylee J Duberstein,Michael J Rothrock,Todd R Callaway,Jin Xie,Franklin D West,Hea Jin Park

Journal of anatomy 242:224-234 PubMed36181694

2022

Mechanisms driving vestibular lamina formation and opening in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Tengyang Qiu,Abigail S Tucker

Acta biochimica et biophysica Sinica 54:1365-1375 PubMed36148952

2022

lncRNA TUG1 regulates hyperuricemia-induced renal fibrosis in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Haizhen Zhang,Langtao Hu,Jiali Wei,Chunyang Ma

International journal of molecular sciences 23: PubMed36142143

2022

TNF-α and IL-1β Modulate Blood-Brain Barrier Permeability and Decrease Amyloid-β Peptide Efflux in a Human Blood-Brain Barrier Model.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Versele,Emmanuel Sevin,Fabien Gosselet,Laurence Fenart,Pietra Candela
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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