Anti-Occludin 抗体 (ab235986)
Key features and details
- Rabbit polyclonal to Occludin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Occludin antibody
Occludin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Occludin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Occludin aa 285-430.
Database link: Q16625 -
ポジティブ･コントロール
- WB: MCF7 whole cell lysate. IHC-P: Human colon cancer tissue. ICC/IF: MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purity >95% -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab235986の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
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| IHC-P |
1/200 - 1/500.
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| WB |
1/500 - 1/5000. Predicted molecular weight: 59 kDa.
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| 特記事項 |
|---|
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ICC/IF
1/50 - 1/200. |
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IHC-P
1/200 - 1/500. |
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WB
1/500 - 1/5000. Predicted molecular weight: 59 kDa. |
ターゲット情報
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機能
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. -
組織特異性
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. -
関連疾患
Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay. -
配列類似性
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain. -
ドメイン
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. -
細胞内局在
Membrane. Cell junction > tight junction. - Information by UniProt
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参照データベース
- Entrez Gene: 100506658 Human
- Omim: 602876 Human
- SwissProt: Q16625 Human
- Unigene: 592605 Human
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別名
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
see all
画像
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Western blot - Anti-Occludin antibody (ab235986)Anti-Occludin antibody (ab235986) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Occludin antibody (ab235986)Paraffin-embedded human colon cancer tissue stained for Occludin using ab235986 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Occludin antibody (ab235986)4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells stained for Occludin (green) using ab235986 at 1/133 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab235986 は 4 報の論文で使用されています。
- Zhao Y et al. Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis. Mol Neurobiol 61:1936-1952 (2024). PubMed: 37819429
- Zhou J et al. The Degradation of Airway Epithelial Tight Junctions in Asthma Under High Airway Pressure Is Probably Mediated by Piezo-1. Front Physiol 12:637790 (2021). PubMed: 33868003
- Tian P et al. Knockdown of ghrelin-O-acyltransferase attenuates colitis through the modulation of inflammatory factors and tight junction proteins in the intestinal epithelium. Cell Biol Int 44:1681-1690 (2020). PubMed: 32281710
- Cui C et al. Intestinal Barrier Breakdown and Mucosal Microbiota Disturbance in Neuromyelitis Optical Spectrum Disorders. Front Immunol 11:2101 (2020). PubMed: 32983166