Anti-OC-3 抗体 [10B2F5] (ab181450)
![Western blot - Anti-OC-3 antibody [10B2F5] (ab181450)](https://www.abcam.co.jp/ps/products/181/ab181450/Images/ab181450-211135-anti-oc-3-antibody-10b2f5-western-blot.jpg "Western blot - Anti-OC-3 antibody [10B2F5] (ab181450)")
Key features and details
- Mouse monoclonal [10B2F5] to OC-3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-OC-3 antibody [10B2F5]
OC-3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10B2F5] to OC-3 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human OC-3 aa 350-455. Expressed in E.coli.
Sequence:C RSQGTLSDLL RNPKPWSKLK SGRETFRRMW KWLQEPEFQR MSALRLAACK RKEQEQQKER ALQPKKQRLV FTDLQRRTLI AIFKENKRPS KEMQVTISQQ LGLEL
Database link: Q60422 -
ポジティブ･コントロール
- Recombinant Human OC-3 protein; OC-3-transfected HEK293 cell lysate; Human cervical cancer tissue; Human rectum cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1 -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
10B2F5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181450の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
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| IHC-P |
1/200 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
|
IHC-P
1/200 - 1/1000. |
ターゲット情報
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機能
Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. -
配列類似性
Contains 1 CUT DNA-binding domain.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 390874 Human
- Entrez Gene: 246086 Mouse
- Omim: 611294 Human
- SwissProt: O60422 Human
- SwissProt: Q8K557 Mouse
- Unigene: 654347 Human
- Unigene: 250572 Mouse
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別名
- OC 3 antibody
- OC-3 antibody
- OC3 antibody
see all
画像
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Western blot - Anti-OC-3 antibody [10B2F5] (ab181450)All lanes : Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : OC-3-transfected HEK293 cell lysate
Predicted band size: 50 kDa -
![Western blot - Anti-OC-3 antibody [10B2F5] (ab181450)](https://www.abcam.co.jp/ps/products/181/ab181450/Images/ab181450-211134-anti-oc-3-antibody-10b2f5-western-blot.jpg)
Western blot - Anti-OC-3 antibody [10B2F5] (ab181450)Anti-OC-3 antibody [10B2F5] (ab181450) at 1/500 dilution + recombinant Human ONECUT3 protein
Predicted band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OC-3 antibody [10B2F5] (ab181450)](https://www.abcam.co.jp/ps/products/181/ab181450/Images/ab181450-211132-anti-oc-3-antibody-10b2f5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OC-3 antibody [10B2F5] (ab181450)Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OC-3 antibody [10B2F5] (ab181450)](https://www.abcam.co.jp/ps/products/181/ab181450/Images/ab181450-211131-anti-oc-3-antibody-10b2f5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OC-3 antibody [10B2F5] (ab181450)Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling OC-3 with ab181450 at a 1/200 dilution.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab181450 は 1 報の論文で使用されています。
- Chen Y et al. Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. Theranostics 12:910-928 (2022). PubMed: 34976220
