AB169083

Anti-NUSAP 抗体

Name: Anti-NUSAP 抗体 (ab169083) | Abcam
Brand: Abcam Limited
SKU: AB169083
Price: 76000 JPY
Availability: InStock

Anti-NUSAP antibody

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(4 Publications)

Mouse Polyclonal NUSAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus.

別名を表示する

ANKT, BM-037, PRO0310, NUSAP1, Nucleolar and spindle-associated protein 1, NuSAP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

Immunofluorescent analysis of HeLa cells labeling NuSAP with ab169083 at 10μg/ml.

Unknown

Western blot - Anti-NUSAP antibody (AB169083)

All lanes:

Western blot - Anti-NUSAP antibody (ab169083) at 1 µg/mL

Lane 1:

NUSAP1-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 49 kDa

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Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.

Biological function summary

NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.

Pathways

NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.

NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.

See full target information NUSAP1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 16:1083-1089 PubMed34322597

2021

Hypoxia stimulates the migration and invasion of osteosarcoma via up-regulating the NUSAP1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhang,Jingtao Song,Xu Xin,Donghong Sun,Huiting Huang,Yang Chen,Tao Zhang,Yiming Zhang

Experimental and therapeutic medicine 20:1145-1152 PubMed32765662

2020

Prazosin inhibits the proliferation, migration and invasion, but promotes the apoptosis of U251 and U87 cells via the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Jiye Fan

Technology in cancer research & treatment 19:1533033820905823 PubMed32233835

2020

Knockdown of lncRNA OGFRP1 Inhibits Proliferation and Invasion of JEG-3 Cells Via AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Meng,Haiyan Xue

Aging 12:2156-2168 PubMed32018225

2020

Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Hao,Kun Pang,Hui Pang,Junjie Zhang,Zhiguo Zhang,Houguang He,Rongsheng Zhou,Zhenduo Shi,Conghui Han

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUSAP 抗体

Anti-NUSAP antibody

Immunocytochemistry/ Immunofluorescence - Anti-NUSAP antibody (AB169083)

Western blot - Anti-NUSAP antibody (AB169083)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise