Anti-NUP98 抗体 [13C2] (ab179894)
Key features and details
- Mouse monoclonal [13C2] to NUP98
- Suitable for: WB, ICC/IF
- Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
- Isotype: IgG1
製品の概要
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製品名
Anti-NUP98 antibody [13C2]
NUP98 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [13C2] to NUP98 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe -
免疫原
This product was produced with the following immunogens:
Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
Sequence:MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ
Database link: D3KYQ3
Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664 (internal sequence).
Sequence: SNPTQGGGLFGAANPGLGG
Database link: D3KYQ3 -
ポジティブ・コントロール
- HeLa cells and cell extract, Tetrahymena themophila cells and cell extract, Saccharomyces cerevisiae cells and cell extract, Schizosaccharomyces pombe cells and cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
13C2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179894の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 112 kDa.
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ICC/IF |
Use a concentration of 0.5 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 112 kDa. |
ICC/IF
Use a concentration of 0.5 µg/ml. |
ターゲット情報
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機能
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
関連疾患
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
配列類似性
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
ドメイン
Contains G-L-F-G repeats. -
翻訳後修飾
Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
細胞内局在
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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参照データベース
- Entrez Gene: 4928 Human
- Entrez Gene: 852788 Saccharomyces cerevisiae
- Entrez Gene: 2541547 Schizosaccharomyces pombe
- Entrez Gene: 7832586 Tetrahymena
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: P49687 Saccharomyces cerevisiae
- SwissProt: Q9UTK4 Schizosaccharomyces pombe
see all -
別名
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
画像
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Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 µg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).
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Anti-NUP98 antibody [13C2] (ab179894) at 0.4 µg/ml + HeLa cell extract
Secondary
HRP-labeled anti-mouse IgG at 0.4 µg/ml
Developed using the ECL technique.
Predicted band size: 112 kDa -
Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 µg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.
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All lanes : Anti-NUP98 antibody [13C2] (ab179894)
Lane 1 : Tetrahymena thermophila wild type cell extract
Lane 2 : Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)
Predicted band size: 112 kDaOpen arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.
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Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.
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All lanes : Anti-NUP98 antibody [13C2] (ab179894)
Lane 1 : S. pombe cell extract
Lane 2 : NUP98-GFP-expressing S. pombe cell extract
Predicted band size: 112 kDa -
Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.
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Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution + S. cerevisiae cell extract
Predicted band size: 112 kDaab179894 crossreacts with multiple nucleoporins of S. cerevisiae.
Asterisks represent uncharacterized proteins.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab179894 は 1 報の論文で使用されています。
- Iwamoto M et al. Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans. Monoclon Antib Immunodiagn Immunother 32:81-90 (2013). PubMed: 23607342