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Anti-NUP98 抗体 [13C2] (ab179894)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)

Key features and details

  • Mouse monoclonal [13C2] to NUP98
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-NUP98 antibody [13C2]
    NUP98 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [13C2] to NUP98

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

  • 免疫原

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
    Sequence:

    MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ


    Database link: D3KYQ3

    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664 (internal sequence).
    Sequence: SNPTQGGGLFGAANPGLGG
    Database link: D3KYQ3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa cells and cell extract, Tetrahymena themophila cells and cell extract, Saccharomyces cerevisiae cells and cell extract, Schizosaccharomyces pombe cells and cell extract.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab179894の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 112 kDa.
ICC/IF
Use a concentration of 0.5 µg/ml.
特記事項
WB
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 112 kDa.
ICC/IF
Use a concentration of 0.5 µg/ml.

ターゲット情報

  • 機能

    Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.

  • 関連疾患

    Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
    Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
    Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
    Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
    Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.

  • 配列類似性

    Belongs to the nucleoporin GLFG family.
    Contains 1 peptidase S59 domain.

  • ドメイン

    Contains G-L-F-G repeats.

  • 翻訳後修飾

    Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.

  • 細胞内局在

    Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 96 kDa nucleoporin antibody
    • 98 kDa nucleoporin antibody
    • ADAR2 antibody
    • ADIR2 antibody
    • GLFG-repeat containing nucleoporin antibody
    • Nuclear pore complex protein Nup96 antibody
    • Nuclear pore complex protein Nup98 Nup96 antibody
    • Nucleoporin 98kD antibody
    • nucleoporin 98kDa antibody
    • Nucleoporin Nup96 antibody
    • Nucleoporin Nup98 antibody
    • NUP196 antibody
    • NUP96 antibody
    • Nup98 antibody
    • Nup98-Nup96 antibody
    • NUP98_HUMAN antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)

    Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 µg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).

  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Anti-NUP98 antibody [13C2] (ab179894) at 0.4 µg/ml + HeLa cell extract

    Secondary
    HRP-labeled anti-mouse IgG at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)

    Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 µg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.

  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    All lanes : Anti-NUP98 antibody [13C2] (ab179894)

    Lane 1 : Tetrahymena thermophila wild type cell extract
    Lane 2 : Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)

    Predicted band size: 112 kDa



    Open arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)

    Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    All lanes : Anti-NUP98 antibody [13C2] (ab179894)

    Lane 1 : S. pombe cell extract
    Lane 2 : NUP98-GFP-expressing S. pombe cell extract

    Predicted band size: 112 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] (ab179894)

    Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] (ab179894)
    Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution + S. cerevisiae cell extract

    Predicted band size: 112 kDa



    ab179894 crossreacts with multiple nucleoporins of S. cerevisiae.

    Asterisks represent uncharacterized proteins.

参考文献 (1)

ab179894 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab179894 は 1 報の論文で使用されています。

  • Iwamoto M  et al. Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans. Monoclon Antib Immunodiagn Immunother 32:81-90 (2013). PubMed: 23607342

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