Anti-NUP88 抗体 (ab79785)
Key features and details
- Rabbit polyclonal to NUP88
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUP88 antibody
NUP88 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NUP88 -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Orangutan
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免疫原
Synthetic peptide corresponding to Human NUP88 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90188) -
ポジティブ･コントロール
- This antibody gave a positive signal in HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Essential component of nuclear pore complex. -
組織特異性
Ubiquitous. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nuclear pore complex. - Information by UniProt
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参照データベース
- Entrez Gene: 4927 Human
- Entrez Gene: 19069 Mouse
- Entrez Gene: 113929 Rat
- Omim: 602552 Human
- SwissProt: Q99567 Human
- SwissProt: Q8CEC0 Mouse
- SwissProt: O08658 Rat
- Unigene: 584784 Human
see all -
別名
- 88 kDa nucleoporin antibody
- karyoporin antibody
- Nuclear pore complex protein 88 antibody
see all
画像
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Western blot - Anti-NUP88 antibody (ab79785)Anti-NUP88 antibody (ab79785) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Nuclear pore complex protein Nup88 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (83 kDa).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab79785 は 6 報の論文で使用されています。
- Iyer SR et al. Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle. Exp Gerontol 150:111338 (2021). PubMed: 33862137
- Lange J et al. Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons. Front Cell Neurosci 15:742763 (2021). PubMed: 34658796
- Fichtman B et al. Pathogenic Variants in NUP214 Cause "Plugged" Nuclear Pore Channels and Acute Febrile Encephalopathy. Am J Hum Genet 105:48-64 (2019). PubMed: 31178128
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
- Grima JC et al. Mutant Huntingtin Disrupts the Nuclear Pore Complex. Neuron 94:93-107.e6 (2017). PubMed: 28384479
- Sugiyama K et al. Calpain-Dependent Degradation of Nucleoporins Contributes to Motor Neuron Death in a Mouse Model of Chronic Excitotoxicity. J Neurosci 37:8830-8844 (2017). PubMed: 28821644