Anti-NUP50 抗体 (ab85915)
Key features and details
- Rabbit polyclonal to NUP50
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NUP50 antibody
NUP50 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NUP50 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide, corresponding to a region between residues 418 and 468 of Human NUP50 (NP_009103.2)
-
ポジティブ･コントロール
- HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85915 was affinity purified using an epitope specific to NUP50 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85915の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. -
組織特異性
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. -
配列類似性
Contains 1 RanBD1 domain. -
ドメイン
Contains FG repeats. -
細胞内局在
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex. - Information by UniProt
-
参照データベース
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- Unigene: 727643 Human
- Unigene: 28379 Mouse
- Unigene: 391486 Mouse
-
別名
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
see all
画像
-
Western blot - Anti-NUP50 antibody (ab85915)All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody (ab85915)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-NUP50 antibody (ab85915)Detection of NUP50 by Western Blot of Immunprecipitate.
ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab85915 は 2 報の論文で使用されています。
- Colussi C et al. Cytoplasmic HDAC4 recovers synaptic function in the 3×Tg mouse model of Alzheimer's disease. Neuropathol Appl Neurobiol 49:e12861 (2023). PubMed: 36331820
- Holzer G et al. The nucleoporin Nup50 activates the Ran guanine nucleotide exchange factor RCC1 to promote NPC assembly at the end of mitosis. EMBO J 40:e108788 (2021). PubMed: 34725842