Anti-NUP214 抗体 (ab70497)
Key features and details
- Rabbit polyclonal to NUP214
- Suitable for: ICC, ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUP214 antibody
NUP214 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NUP214 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: ICC, ICC/IF, IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 1250 and 1300 of human NUP214. (NP_005076.3)
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ポジティブ･コントロール
- WB: HeLa and KEK293T lysate; IP: HeLa lysate; IHC: ovarian carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70497の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19339552
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa).
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| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
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ICC
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 19339552 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 214 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. -
組織特異性
Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development. -
関連疾患
Note=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. -
ドメイン
Contains FG repeats. -
翻訳後修飾
Probably glycosylated as it reacts with wheat germ agglutinin (WGA). -
細胞内局在
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 8021 Human
- Entrez Gene: 227720 Mouse
- Omim: 114350 Human
- SwissProt: P35658 Human
- SwissProt: Q80U93 Mouse
- Unigene: 654530 Human
- Unigene: 289686 Mouse
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別名
- 214 kDa nucleoporin antibody
- CAIN antibody
- CAN antibody
see all
画像
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Western blot - Anti-NUP214 antibody (ab70497)All lanes : Anti-NUP214 antibody (ab70497) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 214 kDa -
Immunoprecipitation - Anti-NUP214 antibody (ab70497)NUP214 was immunoprecipitated from HeLa whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70497 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70497 at 0.04 µg/mL.
Lane 1: ab70497 IP in HeLa whole cell lysate.Lane 2: ab70497 IP in HeLa whole cell lysate.
Lane 2: rabbit anti-NUP214 antibody IP in HeLa whole cell lysate.
Lane 4: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (ab70497)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP214 antibody (ab70497)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma labeling NUP214 with ab70497 at 1/200 dilution.Detection: DAB
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab70497 は 19 報の論文で使用されています。
- Udi Y et al. A general method for quantitative fractionation of mammalian cells. J Cell Biol 222:N/A (2023). PubMed: 36920247
- Cigdem S et al. SET-NUP214 and MLL cooperatively regulate the promoter activity of the HoxA10 gene. Genes Cells 26:830-837 (2021). PubMed: 34320268
- Senbas Akyazi B et al. Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214. Turk J Biol 44:82-92 (2020). PubMed: 32256144
- Lv L et al. Long Non-coding RNA LINC00114 Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression. Front Oncol 9:1383 (2019). PubMed: 31921641
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530