Anti-Nup153 抗体 [QE5]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

ab24700 staining Nup153 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24700 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• ICC/IF

Characteriser

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 4% formaldehyde and permeabilized in 0.2% Triton x100 for 10 minutes at room temperature, then washed 3 times in PBS. The fixation and permeabilization was carried out in a single step. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).

A : HeLa cells, ab24700 used at 4μg/ml
B : 3T3 cells, ab24700 used at 2μg/ml
C : 3T3 cells, ab24700 used at 4μg/ml
D : 3T3 cells, ab24700 used at 6.6μg/ml

This image is courtesy of Patrizia Lavia & Maria de Luca, University La Sapienza

• ICC/IF

Characteriser

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 100% methanol for 20 minutes at -20°C, then washed once in PBS. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).

A : HeLa cells, ab24700 used at 4μg/ml
B : 3T3 cells, ab24700 used at 2μg/ml
C : 3T3 cells, ab24700 used at 4μg/ml
D : 3T3 cells, ab24700 used at 6.6μg/ml

This image is courtesy of Patrizia Lavia & Maria de Luca, University La Sapienza

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

ab24700 staining Nup153 in HeLa cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab24700 at 0.4 μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

Methanol fixed HeLa stained with ab24700. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with Giantin (yellow).

• ICC/IF

AbReview49631****

Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (AB24700)

Immunocytochemical analysis of U2OS cells, labeling Nup153 with ab24700. Cells were formaldehyde fixed, permeabilized with NP40, and blocked with 3% BSA for 1 hour at 21°C. Immunostaining with ab24700 diluted 1/100 for 12 hours at 4°C.

This image is courtesy of an anonymous Abreview.