Anti-NUMB 抗体 (ab4147)
Key features and details
- Goat polyclonal to NUMB
- Suitable for: ELISA, Flow Cyt, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Chicken, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUMB antibody
NUMB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NUMB -
由来種
Goat -
特異性
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. -
アプリケーション
適用あり: ELISA, Flow Cyt, IHC-P, IP, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Chicken, Human
交差が予測される動物種: Cow, Dog
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免疫原
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ポジティブ･コントロール
- WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells; Flow Cyt: A431 cells IHC: Human prostate tissue
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特記事項
This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membraneThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 10 µg/ml.
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| IHC-P | (3) |
Use a concentration of 8 µg/ml.
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| IP | (1) |
Use at an assay dependent concentration.
For 1 hr on mouse brain lysate (see Abreview). |
| WB | (3) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
| ICC/IF | (3) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 8 µg/ml. |
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IP
Use at an assay dependent concentration. For 1 hr on mouse brain lysate (see Abreview). |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
配列類似性
Contains 1 PID domain. -
翻訳後修飾
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 512187 Cow
- Entrez Gene: 480381 Dog
- Entrez Gene: 8650 Human
- Entrez Gene: 18222 Mouse
- Omim: 603728 Human
- SwissProt: P49757 Human
- SwissProt: Q9QZS3 Mouse
- Unigene: 654609 Human
see all -
別名
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
see all
画像
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Western blot - Anti-NUMB antibody (ab4147)Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)Immunohistochemistry staining of paraffin embedded Human Prostate labeling NUMB with ab4147 at (8µg/mL). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Flow Cytometry - Anti-NUMB antibody (ab4147)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreviewab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (41)
ab4147 は 41 報の論文で使用されています。
- Herrejon Chavez F et al. RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells. Nat Commun 14:2290 (2023). PubMed: 37085479
- Li MR et al. MAD2B promotes podocyte injury through regulating Numb-dependent Notch 1 pathway in diabetic nephropathy. Int J Biol Sci 18:1896-1911 (2022). PubMed: 35342338
- Charruyer A et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell 20:e13310 (2021). PubMed: 33524216
- Lau EO et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife 10:N/A (2021). PubMed: 33899739
- Gomez K et al. Non-SUMOylated CRMP2 decreases NaV1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15. Mol Brain 14:20 (2021). PubMed: 33478555