Anti-NUDC 抗体 [EPR5073] (ab109318)
![Western blot - Anti-NUDC antibody [EPR5073] (ab109318)](https://www.abcam.co.jp/ps/products/109/ab109318/Images/ab109318-122092-anti-nudc-antibody-epr5073-western-blot.jpg "Western blot - Anti-NUDC antibody [EPR5073] (ab109318)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5073] to NUDC
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NUDC antibody [EPR5073]
NUDC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5073] to NUDC -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2, 293T, HeLa and SH-SY5Y cell lysates IHC-P: Human brain tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5073 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation. -
組織特異性
Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart. -
配列類似性
Belongs to the nudC family.
Contains 1 CS domain. -
翻訳後修飾
Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases. -
細胞内局在
Cytoplasm > cytoskeleton. Nucleus. In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern. - Information by UniProt
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参照データベース
- Entrez Gene: 10726 Human
- Entrez Gene: 18221 Mouse
- Entrez Gene: 29648 Rat
- Omim: 610325 Human
- SwissProt: Q9Y266 Human
- SwissProt: O35685 Mouse
- SwissProt: Q63525 Rat
- Unigene: 263812 Human
see all -
別名
- HNUDC antibody
- MNUDC antibody
- MNUDC protein antibody
see all
画像
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Western blot - Anti-NUDC antibody [EPR5073] (ab109318)All lanes : Anti-NUDC antibody [EPR5073] (ab109318) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : HeLa lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDC antibody [EPR5073] (ab109318)](https://www.abcam.co.jp/ps/products/109/ab109318/Images/ab109318-122091-anti-nudc-antibody-epr5073-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDC antibody [EPR5073] (ab109318)Immunohistochemical analysis of NUDC in paraffin embedded Human brain tissue, using ab109318 at a 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-NUDC antibody [EPR5073] (ab109318)](https://www.abcam.co.jp/ps/products/109/ab109318/Images/ab109318-3-benefits-of-recombinant-antibodies.png)
Anti-NUDC antibody [EPR5073] (ab109318)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab109318 は論文での使用が確認できていません。