AB248337

Anti-Nucleostemin 抗体 [EPR3911(2)] - BSA and Azide free

Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nucleostemin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

別名を表示する

E2IG3, NS, GNL3, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, Nucleolar GTP-binding protein 3, Nucleostemin, NNP47

3 Images

Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

This data was developed using ab129185, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SW480 cells using ab129185 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

This data was developed using ab129185, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nucleostemin antibody [EPR3911(2)] (<a href='/products/primary-antibodies/nucleostemin-antibody-epr39112-ab129185'>ab129185</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

PC-3 cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 62 kDa

false

OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3911(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

ab248337 is the carrier-free version of ab129185.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nucleostemin is a protein involved in cellular regulation and has an approximate mass of 60 kDa. It is also known by the alternative name GNL3. Mechanical function of nucleostemin involves involvement in the regulation of cell cycle and maintenance of stem cell properties. This protein is expressed mainly in the nucleolus and is found in abundance within stem cells and certain cancer cells where its levels change depending on cell cycle stages.

Biological function summary

Nucleostemin plays an important role in controlling cell proliferation and maintaining stem cell characteristics. It is not part of a large complex but interacts with other proteins to perform its functions. The protein facilitates the regulation of cell division and its presence in stem cells assures the maintenance of an undifferentiated state which is important for tissue regeneration and repair.

Pathways

Nucleostemin interacts with various cell cycle and DNA repair mechanisms. Among important biological pathways nucleostemin is involved in the p53 signaling pathway assisting in cellular stress responses and enabling cell survival during DNA damage. There is also a relationship with the MDM2 protein as nucleostemin indirectly influences p53 stability by modulating MDM2 activity therefore playing a role in tumor suppression and oncogenic processes.

Researchers associate nucleostemin with cancer and neurological disorders. In cancers its overexpression is linked to the unchecked proliferation of tumor cells often observed in glioblastoma and certain sarcomas where nucleostemin interacts with proteins like p53 and MDM2 to influence tumor dynamics. In neurological contexts its role in stem cell regulation draws attention when considering diseases involving neurodegeneration where maintaining neural stem cells remains important.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

See full target information GNL3

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleostemin 抗体 [EPR3911(2)] - BSA and Azide free

Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

Western blot - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

OI-RD Scanning - Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free (AB248337)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise