Anti-Nucleoside phosphorylase 抗体 [EPR5714] (ab109559)
![Western blot - Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)](https://www.abcam.co.jp/ps/products/109/ab109559/Images/ab109559-393575-anti-nucleoside-phosphorylase-antibody-epr5714-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5714] to Nucleoside phosphorylase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nucleoside phosphorylase antibody [EPR5714]
Nucleoside phosphorylase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5714] to Nucleoside phosphorylase -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat, JAR, 293T and HeLa cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5714 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 32 kDa. |
ターゲット情報
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関連疾患
Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children. -
配列類似性
Belongs to the PNP/MTAP phosphorylase family. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 4860 Human
- Omim: 164050 Human
- SwissProt: P00491 Human
- Unigene: 75514 Human
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別名
- FLJ94043 antibody
- FLJ97288 antibody
- FLJ97312 antibody
see all
画像
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Western blot - Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)All lanes : Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PNP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab109559 observed at 31 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109559 Anti-Nucleoside phosphorylase antibody [EPR5714] was shown to specifically react with Nucleoside phosphorylase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266158 (knockout cell lysate ab257594) was used. Wild-type and Nucleoside phosphorylase knockout samples were subjected to SDS-PAGE. ab109559 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)](https://www.abcam.co.jp/ps/products/109/ab109559/Images/Nucleoside-phosphorylase-Primary-antibodies-ab109559-1.jpg)
Western blot - Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)All lanes : Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : JAR cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa -
![Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)](https://www.abcam.co.jp/ps/products/109/ab109559/Images/ab109559-3-benefits-of-recombinant-antibodies.png)
Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109559 は 1 報の論文で使用されています。
- Tang DH et al. Inosine induces acute hyperuricaemia in rhesus monkey (Macaca mulatta) as a potential disease animal model. Pharm Biol 59:175-182 (2021). PubMed: 33715593