AB228609

Anti-Nucleoside-diphosphate kinase 抗体

Anti-Nucleoside-diphosphate kinase antibody

JP Deleterious:医薬用外劇物

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Rabbit Polyclonal Nucleoside-diphosphate kinase antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CMPK1.

別名を表示する

CMK, CMPK, UCK, UMK, UMPK, CMPK1, UMP-CMP kinase, Deoxycytidylate kinase, Nucleoside-diphosphate kinase, Uridine monophosphate/cytidine monophosphate kinase, CK, dCMP kinase, UMP/CMP kinase, UMP/CMPK

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

HCT 116 (human colorectal carcinoma cell line) cells stained for Nucleoside-diphosphate kinase (green) using ab228609 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

12% SDS-PAGE

All lanes:

Western blot - Anti-Nucleoside-diphosphate kinase antibody (ab228609) at 1/500 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 22 kDa

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Supplier Data

Western blot - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

12% SDS-PAGE

All lanes:

Western blot - Anti-Nucleoside-diphosphate kinase antibody (ab228609) at 1/1000 dilution

All lanes:

Mouse brain tissue whole cell lysate at 50 µg

Predicted band size: 22 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human CMPK1. The exact immunogen used to generate this antibody is proprietary information.

P30085

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside-diphosphate kinase (NDPK) also known as nucleoside-diphosphate kinase NM23 and NDP kinase mechanically catalyzes the exchange of phosphate groups between different nucleoside diphosphates and triphosphates. This protein weighs approximately 17 kDa and is expressed in various tissues including liver heart and brain. Its catalytic function relies on a ping-pong mechanism where a high-energy phosphoryl transfer occurs important for maintaining the balance of cellular nucleotide pools.

Biological function summary

Nucleoside-diphosphate kinase facilitates essential cellular processes by regulating the levels of nucleoside triphosphates key building blocks for nucleic acid synthesis. It is often found as part of a larger complex composed of hexameric structures in cells impacting cellular energy homeostasis and metabolism. The protein's role extends to involvement in cellular signaling differentiation and proliferation contributing to its importance in cellular regulation.

Pathways

The role of nucleoside-diphosphate kinase extends to critical biological pathways such as the nucleotide metabolism and cell signaling pathways where it ensures the equilibrium of nucleoside triphosphates used in DNA and RNA synthesis. NDPK interacts with other proteins particularly linking with kinases and phosphatases to perform its regulatory functions. This protein integrates closely with the MAPK signaling pathway influencing cellular responses to external stimuli and cellular growth.

Issues with nucleoside-diphosphate kinase have been linked to conditions such as cancer and neurodegenerative diseases. Mutations or aberrant expression of NDPK can impact its function leading to alterations in cellular processes that drive oncogenesis. For example its altered interaction with proteins like p53 in cancer can impair tumor suppressor functions facilitating tumorigenesis. Additionally its dysregulation has associations with neurodegenerative diseases where it may affect neuronal survival and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.

See full target information CMPK1

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Nucleoside-diphosphate kinase 抗体

Anti-Nucleoside-diphosphate kinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

Western blot - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

Western blot - Anti-Nucleoside-diphosphate kinase antibody (AB228609)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Abcam product promise