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リコンビナントRabMAb

Anti-Nucleophosmin 抗体 [SP236] - BSA and Azide free (ab245744)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
  • Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP236] to Nucleophosmin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Nucleophosmin antibody [SP236] - BSA and Azide free
    Nucleophosmin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP236] to Nucleophosmin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil, Human skin, Mouse colon, and Rat spleen tissue; Flow Cyt (intra): HeLa and 3T3-L1 cells; ICC: 3T3-L1, HeLa and PC-12 cells.

  • 特記事項

    ab245744 is the carrier-free version of ab183340.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • 特記事項（精製）

    Purified from TCS by protein A/G.

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP236

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245744の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.

ターゲット情報

  • 機能

    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).

  • 関連疾患

    A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
    A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
    A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
    Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.

  • 配列類似性

    Belongs to the nucleoplasmin family.

  • 翻訳後修飾

    Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
    ADP-ribosylated.
    Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
    Sumoylated by ARF.

  • 細胞内局在

    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • B23 antibody
    • MGC104254 antibody
    • NO38 antibody
    • NPM antibody
    • NPM_HUMAN antibody
    • NPM1 antibody
    • Nucleolar phosphoprotein B23 antibody
    • Nucleolar protein NO38 antibody
    • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
    • Nucleophosmin antibody
    • nucleophosmin nucleoplasmin family member 1 antibody
    • Nucleophosmin/nucleoplasmin family member 1 antibody
    • Numatrin antibody
    • OTTHUMP00000161024 antibody
    • OTTHUMP00000161025 antibody
    • OTTHUMP00000223397 antibody
    • OTTHUMP00000223398 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Nucleophosmin with ab183340 at 1/100 dilution (2.51 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleophosmin with ab183340 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183340).

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling Nucleophosmin with purified ab183340 at 1/100(2.5µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

  • Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (mouse embryonic fibroblast) cells labeling Nucleophosmin with purified ab183340 at 1/100 (2.5µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340).

  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Intracellular Flow Cytometry analysis of 3T3-L1 (Mouse embryonic fibroblast) labeling Nucleophosmin with purified ab183340 at 1/200 dilution (1.255µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183340). 

     

     

  • Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Flow Cytometry (Intracellular) - Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

    Intracellular flow cytometric analysis of Jurkat cells, labeling Nucleophosmin with ab183340diluted 1/400 (green), compared with a negative control of anti-rabbit IgG (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183340). 

     

     

  • Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)
    Anti-Nucleophosmin antibody [SP236] - BSA and Azide free (ab245744)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab245744 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab245744 は論文での使用が確認できていません。

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com