Anti-Nucleolin 抗体 (ab50279)
Key features and details
- Rabbit polyclonal to Nucleolin
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Nucleolin antibody
Nucleolin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nucleolin -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cynomolgus monkey, Orangutan
-
免疫原
Synthetic peptide:
VKLAKAGKNQGDPKKMA
conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody has been affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50279の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration. PubMed: 20814900
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use a concentration of 10 - 20 µg/ml.
|
|
| IP |
Use at an assay dependent concentration.
1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells. |
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. PubMed: 20814900 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 10 - 20 µg/ml. |
|
IP
Use at an assay dependent concentration. 1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells. |
ターゲット情報
-
機能
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
翻訳後修飾
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
-
参照データベース
- Entrez Gene: 101865794 Cynomolgus monkey
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 100174230 Orangutan
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: Q4R4J7 Cynomolgus monkey
- SwissProt: P19338 Human
see all -
別名
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (ab50279)Indirect Immunofluorescence
Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (30)
ab50279 は 30 報の論文で使用されています。
- Laspata N et al. PARP1 associates with R-loops to promote their resolution and genome stability. Nucleic Acids Res 51:2215-2237 (2023). PubMed: 36794853
- Marchena-Cruz E et al. DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops. Cell Rep 42:112148 (2023). PubMed: 36827184
- Bayona-Feliu A et al. The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts. Nat Commun 14:6890 (2023). PubMed: 37898641
- Lappin KM et al. Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors. Cancer Res 82:819-830 (2022). PubMed: 35027467
- Hao S et al. Dynamic switching of crotonylation to ubiquitination of H2A at lysine 119 attenuates transcription-replication conflicts caused by replication stress. Nucleic Acids Res 50:9873-9892 (2022). PubMed: 36062559