Anti-Nucleolin 抗体 [3G4B2] (ab14966)
Key features and details
- Mouse monoclonal [3G4B2] to Nucleolin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nucleolin antibody [3G4B2]
Nucleolin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3G4B2] to Nucleolin -
由来種
Mouse -
特異性
Recognizes human nucleolin, MW 100kD and an additional protein, MW 95kDa in HeLa nuclear extract. The clone number has been updated from (4i51) to (3G4B2) both clone numbers name the same antibody clone. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Nuclear matrix extract from MDCK cells (dog).
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ポジティブ・コントロール
- WB: HeLa nuclear extract. IP: MDCK total cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 30% Glycerol
Supplied as a liquid in 100µl of culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
3G4B2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa).
A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions. |
特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa). A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions. |
ターゲット情報
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機能
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
翻訳後修飾
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
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別名
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab14966 は 5 報の論文で使用されています。
- Baez M et al. Analysis of the small chromosomal Prionium serratum (Cyperid) demonstrates the importance of reliable methods to differentiate between mono- and holocentricity. Chromosoma 129:285-297 (2020). PubMed: 33165742
- Wang Y et al. PNRC accumulates in the nucleolus by interaction with B23/nucleophosmin via its nucleolar localization sequence. Biochim Biophys Acta : (2010). PubMed: 20888865
- Hwang J et al. Role of Scarf and its binding target proteins in epidermal calcium homeostasis. J Biol Chem 282:18645-53 (2007). IHC-P, IP . PubMed: 17470426
- Huddleson JP et al. Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin. J Biol Chem 281:15121-8 (2006). WB, EMSA ; Human . PubMed: 16571724
- Gotzmann J et al. Two-dimensional electrophoresis reveals a nuclear matrix-associated nucleolin complex of basic isoelectric point. Electrophoresis 18:2645-53 (1997). IP ; Human . PubMed: 9527495