Anti-NUCB1 抗体 (ab154262)
Key features and details
- Rabbit polyclonal to NUCB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUCB1 antibody
NUCB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NUCB1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment corresponding to Human NUCB1 aa 224-461.
Database link: Q02818 -
ポジティブ･コントロール
- A549, HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. -
組織特異性
Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas. -
配列類似性
Belongs to the nucleobindin family.
Contains 2 EF-hand domains. -
ドメイン
The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 4924 Human
- Entrez Gene: 18220 Mouse
- Entrez Gene: 84595 Rat
- Omim: 601323 Human
- SwissProt: Q02818 Human
- SwissProt: Q02819 Mouse
- SwissProt: Q63083 Rat
- Unigene: 631602 Human
see all -
別名
- CALNUC antibody
- FLJ40471 antibody
- NUC antibody
see all
画像
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Western blot - Anti-NUCB1 antibody (ab154262)Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-NUCB1 antibody (ab154262)Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (ab154262)Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (ab154262)Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154262 は 2 報の論文で使用されています。
- Huang Y et al. Identification of MKNK1 and TOP3A as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments. Comput Struct Biotechnol J 21:1510-1522 (2023). PubMed: 36851918
- Hua YQ et al. NUCB1 Suppresses Growth and Shows Additive Effects With Gemcitabine in Pancreatic Ductal Adenocarcinoma via the Unfolded Protein Response. Front Cell Dev Biol 9:641836 (2021). PubMed: 33855021