AB154262

Anti-NUCB1 抗体

Name: Anti-NUCB1 抗体 (ab154262) | Abcam
Brand: Abcam Limited
SKU: AB154262
Price: 78000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-NUCB1 antibody

(1 Review)

(3 Publications)

Rabbit Polyclonal NUCB1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus.

別名を表示する

NUC, NUCB1, Nucleobindin-1, CALNUC

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (AB154262)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (AB154262)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (AB154262)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (AB154262)

Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.

Unknown

Western blot - Anti-NUCB1 antibody (AB154262)

10% SDS PAGE

All lanes:

Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 54 kDa

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Western blot - Anti-NUCB1 antibody (AB154262)

10% SDS PAGE

All lanes:

Western blot - Anti-NUCB1 antibody (ab154262) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 54 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human NUCB1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein NUCB1 also known as Nucleobindin-1 functions mechanically as a calcium-binding protein. It has approximately a mass of 55 kDa and belongs to a group of calcium-binding proteins containing EF-hand motifs. NUCB1 expresses in a variety of tissues notably the nervous system and endocrine tissues. The protein comprises several functional domains including a signal peptide helix-loop-helix motif and nuclear localization signal.

Biological function summary

NUCB1 participates in the regulation of cellular calcium homeostasis and signaling pathways. It is not a part of a multi-protein complex but it acts as a molecular chaperone influencing the stability and function of other proteins within the cell. NUCB1 also plays a role in secretory pathways within the cell which is relevant to its expression in endocrine tissues. Its regulatory functions in calcium signaling are critical for cellular activities such as secretion and cell division.

Pathways

NUCB1 interacts with cellular signaling mechanisms particularly those related to intracellular calcium dynamics. It is involved in pathways like the calcium signaling pathway and the insulin signaling pathway. Within these pathways NUCB1 associates with proteins such as calmodulin which also binds calcium and contributes to signal transduction. The protein modulates activities within these pathways affecting how cells respond to external signals.

NUCB1 exhibits associations with certain conditions linked to disturbed calcium regulation. One such disorder is Alzheimer's disease where alterations in calcium signaling play a role in disease progression. The protein furthermore relates to type 2 diabetes involving its role in the insulin signaling pathway. In these contexts NUCB1 connects to other proteins such as amyloid precursor protein in Alzheimer's disease highlighting disruptions in calcium homeostasis mechanisms.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).

See full target information NUCB1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 51:102185 PubMed39522303

2024

Prognostic significance of combined PCPE-1 and α-fetoprotein for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhua Zhang,Wanqi Chen,Xuelan Peng,Zhiguang Zhang,Shangjiu Yang,Li Zhong

Computational and structural biotechnology journal 21:1510-1522 PubMed36851918

2023

Identification of and as ovarian endometriosis risk-associated genes using integrative genomic analyses and functional experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Huang,Jie Luo,Yue Zhang,Tao Zhang,Xiangwei Fei,Liqing Chen,Yingfan Zhu,Songyue Li,Caiyun Zhou,Kaihong Xu,Yunlong Ma,Jun Lin,Jianhong Zhou

Frontiers in cell and developmental biology 9:641836 PubMed33855021

2021

NUCB1 Suppresses Growth and Shows Additive Effects With Gemcitabine in Pancreatic Ductal Adenocarcinoma via the Unfolded Protein Response.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Qiang Hua,Ke Zhang,Jie Sheng,Zhou-Yu Ning,Ye Li,Wei-Dong Shi,Lu-Ming Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NUCB1 抗体

Anti-NUCB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (AB154262)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (AB154262)

Western blot - Anti-NUCB1 antibody (AB154262)

Western blot - Anti-NUCB1 antibody (AB154262)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Assay Kits

Primary Antibodies

Proteins & Peptides

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise