Anti-NSP 5 alpha 3 alpha 抗体 (ab87801)
Key features and details
- Rabbit polyclonal to NSP 5 alpha 3 alpha
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NSP 5 alpha 3 alpha antibody -
製品の詳細
Rabbit polyclonal to NSP 5 alpha 3 alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 119 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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組織特異性
Highly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines. -
関連疾患
Note=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB. -
配列類似性
Belongs to the cytospin-A family.
Contains 1 CH (calponin-homology) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 92521 Human
- Omim: 608793 Human
- SwissProt: Q5M775 Human
- Unigene: 431045 Human
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別名
- Cytokinesis and spindle organization B antibody
- Cytospin B antibody
- Cytospin-B antibody
see all
画像
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Western blot - Anti-NSP 5 alpha 3 alpha antibody (ab87801)All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 119 kDa
Observed band size: 119 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-NSP 5 alpha 3 alpha antibody (ab87801)Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab87801 は論文での使用が確認できていません。