Anti-NRXN2 抗体 (ab34245)

Thank you for your enquiry regarding ab34245 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

The expected band size for mature Neurexin II alpha should be around 185 kDa. The band observed at 50 kDa either represent some degradation product or different splice variants. Since RIPA buffer has been used for sample preparation, as long as the proteinase inhibitors are freshly prepared before the experiments, the degradation can be ruled out.

It is worth mentioning that cells in primary culture tend to dedifferentiate very quickly and the protein expression profile may be different from cells isolated from tissues or from well established cell cultures.

As our datasheet indicates, normal adult human cerebral cortex SKNSH cell line and murine N2A cells were used to test this antibody. It would be advisable to run a good positive control alongside with the samples to compare the expression pattern of Neurexin II alpha.

I would also suggest checking the literature what type isoforms are reported to be expressed in rat hippocampal cells. According to Swiss-Prot database, 18 (!) different isoforms produced by alternative promoter usage and alternative splicing. Please visit this site for further information:

http://www.uniprot.org/uniprot/Q63374

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your enquiry regarding ab34245.

According to Swiss-Prot database, there seems to be some splice variants and isoforms Neurexin II alpha. You may wish to take a look at this site for further information:

http://www.uniprot.org/uniprot/Q9P2S2

Could you please provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Thank you very much for your interest in ab34245. To our knowledge, ab34245 has not been tested in Rat (it is only predicted). Therefore, I can offer a discount off a future purchase if you buy ab34245 now, test it in Rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab34245 in Rat. I will then send a discount code. This code must be issued before purchasing ab34245 so please wait for my reply before ordering. 2. Purchase ab34245 either by phone, fax, or online (www.abcam.com). 3. Test it in Rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34245 turns out to be unsuitable for Rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.