Anti-NRP2 抗体 (ab185710)
Key features and details
- Rabbit polyclonal to NRP2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NRP2 antibody
NRP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NRP2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
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ポジティブ･コントロール
- WB: A-549, HT-1080 cell lysates, mouse kidney tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.05% Proclin 300
Constituents: 49.95% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 105 kDa.Can be blocked with Recombinant Human NRP2 protein (ab160214).
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| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 105 kDa.Can be blocked with Recombinant Human NRP2 protein (ab160214). |
ターゲット情報
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機能
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. -
配列類似性
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
ドメイン
The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8828 Human
- Entrez Gene: 18187 Mouse
- Omim: 602070 Human
- SwissProt: O60462 Human
- SwissProt: O35375 Mouse
- Unigene: 471200 Human
- Unigene: 266341 Mouse
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別名
- MGC126574 antibody
- Neuropilin 2 antibody
- Neuropilin-2 antibody
see all
画像
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Western blot - Anti-NRP2 antibody (ab185710)All lanes : Anti-NRP2 antibody (ab185710) at 1/1000 dilution
Lane 1 : A-549 cell lysate
Lane 2 : HT-1080 cell lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab185710 は 6 報の論文で使用されています。
- Luo X et al. Vascular NRP2 triggers PNET angiogenesis by activating the SSH1-cofilin axis. Cell Biosci 10:113 (2020). PubMed: 32983407
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
- Zhang H et al. miR-331-3p suppresses cell invasion and migration in colorectal carcinoma by directly targeting NRP2. Oncol Lett 18:6501-6508 (2019). PubMed: 31807170
- Manetti M et al. Systemic Sclerosis Serum Significantly Impairs the Multi-Step Lymphangiogenic Process: in Vitro Evidence. Int J Mol Sci 20:N/A (2019). PubMed: 31817940
- Dong X et al. Elevated expression of neuropilin-2 associated with unfavorable prognosis in hepatocellular carcinoma. Onco Targets Ther 10:3827-3833 (2017). PubMed: 28814881
- Huang R et al. Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts. J Transl Med 15:203 (2017). IHC ; Mouse . PubMed: 29017512