Anti-NRG3 抗体 (ab77597)
Key features and details
- Goat polyclonal to NRG3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NRG3 antibody
NRG3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NRG3 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Chimpanzee
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免疫原
Synthetic peptide:
RNEIQRDSALTK
, corresponding to amino acids 685-696 of Human NRG3 (NP_001010848.2) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/64000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. -
組織特異性
Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. -
配列類似性
Belongs to the neuregulin family.
Contains 1 EGF-like domain. -
発生段階
Isoform 3 is expressed in fetal brain but not in other fetal tissues. -
ドメイン
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
ERBB receptor binding is elicited entirely by the EGF-like domain. -
翻訳後修飾
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated. -
細胞内局在
Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form. - Information by UniProt
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参照データベース
- Entrez Gene: 10718 Human
- Omim: 605533 Human
- SwissProt: P56975 Human
- Unigene: 125119 Human
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別名
- HRG3 antibody
- Neuregulin 3 antibody
- Neuregulin 3 like polypeptide antibody
see all
画像
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Western blot - Anti-NRG3 antibody (ab77597)Anti-NRG3 antibody (ab77597) at 0.3 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg
Predicted band size: 78 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Note: The additional 37 kDa band of unknown identity was consistently observed and was successfully blocked by incubation with the immunizing peptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab77597 は 1 報の論文で使用されています。
- Paterson C et al. Temporal Dynamics of the Neuregulin-ErbB Network in the Murine Prefrontal Cortex across the Lifespan. Cereb Cortex 30:3325-3339 (2020). PubMed: 31897479