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Anti-NrCAM 抗体 [S364-51] (ab186247)

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Western blot - Anti-NrCAM antibody [S364-51] (ab186247)
  • Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] (ab186247)

Key features and details

  • Mouse monoclonal [S364-51] to NrCAM
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-NrCAM antibody [S364-51]
    NrCAM 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [S364-51] to NrCAM

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Recombinant fragment within Mouse NrCAM aa 1-850. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (~50% identity with Neurofascin).
    Database link: Q810U4

  • ポジティブ･コントロール

    • Rat brain lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    S364-51

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186247の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 150-160 kDa (predicted molecular weight: 144 kDa).
ICC/IF
1/100.
特記事項
WB
1/1000. Detects a band of approximately 150-160 kDa (predicted molecular weight: 144 kDa).
ICC/IF
1/100.

ターゲット情報

  • 機能

    Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.

  • 組織特異性

    Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Bravo antibody
      • hBravo antibody
      • Neuronal cell adhesion molecule antibody
      • Neuronal surface protein Bravo antibody
      • Ng CAM related antibody
      • Ng-CAM-related antibody
      • NgCAM related cell adhesion molecule antibody
      • NgCAM-related cell adhesion molecule antibody
      • Nr CAM antibody
      • Nr-CAM antibody
      • Nrcam antibody
      • NRCAM_HUMAN antibody
      see all

    画像

    • Western blot - Anti-NrCAM antibody [S364-51] (ab186247)
      Western blot - Anti-NrCAM antibody [S364-51] (ab186247)

      Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (ab186247). Load: 10 µg. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (ab186247) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.

    • Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] (ab186247)
      Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] (ab186247)

      Ab186247 staining NrCAM in SK-N-BE (Human neuroblastoma cell line) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature. Samples were incubated with primary antibody at 1:100 dilution for 60 minutes at room temperature. An Atto 488 Goat anti-mouse was used as the secondary antibody at 1/100 dilution for 60 minutes at room temperature. Phalloidin Texas Red F-Actin was used as a counterstain. DAPI (blue) was used as a nuclear stain (A). Phalloidin Texas Red F-Actin stain (B), ab186247 (C) and merged (D).

    参考文献 (0)

    ab186247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186247 は論文での使用が確認できていません。

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