AB191418

Anti-NrCAM 抗体 [EPR15654] - Neuronal Marker

Anti-NrCAM antibody [EPR15654] - Neuronal Marker

BOND RX™ Validated
RabMAb
Recombinant
Lab Essentials
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(4 Publications)

Rabbit Recombinant Monoclonal NRCAM antibody. Neuron marker. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 4 publications.

別名を表示する

KIAA0343, NRCAM, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, hBravo, Ng-CAM-related

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling NrCAM with ab191418 at 1/500 dilution (0.89 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody at a ready to use concentration. Negative control : no staining on human liver. The section was incubated with ab191418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling NrCAM with ab191418 at 1/500 dilution (0.89 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody at a ready to use concentration. Positive staining on human cerebrum. The section was incubated with ab191418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Western blot analysis of immunoprecipitation pellet from Human glioma lysate immunoprecipitated using ab191418 at 1/30 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (ab191418)

Predicted band size: 144 kDa

false

Supplier Data

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

All lanes:

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (ab191418) at 1/10000 dilution

All lanes:

Human forebrain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 144 kDa

false

Supplier Data

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

All lanes:

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (ab191418) at 1/50000 dilution

All lanes:

Human glioma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 144 kDa,30 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15654

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.

Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.

See full target information NRCAM

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 17:6112-6126 PubMed40950871

2025

The impact of neuron-related cell adhesion molecule on cellular biological functions and its clinical significance in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Peilong Liu,Yan Wang,Hong Xu

Scandinavian journal of immunology 100:e13401 PubMed39155774

2024

NrCAM activates the NF-κB signalling pathway by competitively binding to SUMO-1 and promotes Th17 cell differentiation in Graves' disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjiao Huang,Lijuan Zhang,Yingying Zhou,Shuiying Zhao,Jiao Wang

Journal of Cancer 13:2029-2039 PubMed35399713

2022

KLF9 regulates miR-338-3p/NRCAM axis to block the progression of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlu Liu,Kuijing Han,Yulin Cao,Yuxiang Hu,Zengwu Shao,Wei Tong,Yanjiu Han,Yong Liu

Molecular & cellular proteomics : MCP 20:100041 PubMed33639418

2021

Interspecies Differences in Proteome Turnover Kinetics Are Correlated With Life Spans and Energetic Demands.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Swovick,Denis Firsanov,Kevin A Welle,Jennifer R Hryhorenko,John P Wise,Craig George,Todd L Sformo,Andrei Seluanov,Vera Gorbunova,Sina Ghaemmaghami

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NrCAM 抗体 [EPR15654] - Neuronal Marker

Anti-NrCAM antibody [EPR15654] - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Immunoprecipitation - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (AB191418)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise