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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-NRAS 抗体 [EPR25186-24] - BSA and Azide free (ab300432)

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Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
  • Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
  • Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
  • Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
  • Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25186-24] to NRAS - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-NRAS antibody [EPR25186-24] - BSA and Azide free
    NRAS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25186-24] to NRAS - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: Wild-type HEK-293T, MCF7 (human breast adenocarcinoma epithelial cell), A375 (human malignant melanoma epithelial cell) , C6 (rat glial tumor glial cell), NIH/3T3 (mouse embryonic fibroblast) . Tissue lysates: Rat and mouse spleen. His-tagged human NRAS recombinant protein. IP: MCF7 (human breast adenocarcinoma epithelial cell).

  • 特記事項

    ab300432 is the carrier-free version of ab300431.

    ab300431 does not react in: IHC-P with human, mouse , and rat; ICC with human, and mouse, and intrecellular flow cytometry with mouse. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300432の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

  • 関連疾患

    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.

  • 配列類似性

    Belongs to the small GTPase superfamily. Ras family.

  • 翻訳後修飾

    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.

  • 細胞内局在

    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

画像

  • Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    All lanes : Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1 : Human KRAS recombinant protein, 10 ng
    Lane 2 : His-tagged human HRAS recombinant protein, 10 ng
    Lane 3 : His-tagged human NRAS recombinant protein, 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Observed band size: 21 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    This data was developed using 300431, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human KRAS or HRAS. 

  • Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    All lanes : Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : A375 (human malignant melanoma epithelial cell) whole cell lysate
    Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 6 : Mouse spleen tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 21 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using 300431, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    Western blot - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    All lanes : Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (ab255449) whole cell lysate
    Lane 2 : NRAS knockout HEK-293T (ab266684) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Exposure time: 180 seconds


    This data was developed using 300431, the same antibody clone in a different buffer formulation. 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    In Western blot, ab300431 was shown to bind specifically to NRAS. One band was observed at 21 kDa in wild-type HEK-293T (ab255449) cell lysates with no signal observed at this size in NRAS knockout cell lysates (ab258542).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)

    This data was developed using ab300431, the same antibody clone in a different buffer formulation.

    NRAS was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab300431 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300431 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: ab300431 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300431 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)
    Anti-NRAS antibody [EPR25186-24] - BSA and Azide free (ab300432)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300432 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300432 は論文での使用が確認できていません。

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