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  1. Link

    products/primary-antibodies/nras-antibody-ab77392.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-NRAS 抗体 (ab77392)

  • Datasheet
  • SDS
Reviews (11) Submit a question References (13)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-NRAS antibody (ab77392)

    Key features and details

    • Goat polyclonal to NRAS
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Ras antibody [EPR3255] (ab108602)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-NRAS antibody
      NRAS 一次抗体 製品一覧
    • 製品の詳細

      Goat polyclonal to NRAS
    • 由来種

      Goat
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat
    • 免疫原

      Synthetic peptide within Human NRAS aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession Number: NP_002515.1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • ポジティブ・コントロール

      • WB: MCF7 cell lysate.
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, 0.5% Tris buffered saline
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      ab77392 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Small G Proteins
      • Ras Family
      • Cancer
      • Signal transduction
      • G protein signaling
      • Small G proteins
      • Ras family
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Signal transducers

    関連製品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • A-431 membrane extract lysate (ab30134)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab77392の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (6)
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation at room temperature is recommended for this product.

    特記事項
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation at room temperature is recommended for this product.

    ターゲット情報

    • 機能

      Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • 関連疾患

      Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
      Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
    • 配列類似性

      Belongs to the small GTPase superfamily. Ras family.
    • 翻訳後修飾

      Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
    • 細胞内局在

      Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
    • Target information above from: UniProt accession P01111 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 4893 Human
      • Entrez Gene: 18176 Mouse
      • Entrez Gene: 24605 Rat
      • Omim: 164790 Human
      • SwissProt: P01111 Human
      • SwissProt: P08556 Mouse
      • SwissProt: Q04970 Rat
      • Unigene: 486502 Human
      • Unigene: 400954 Mouse
      • Unigene: 217722 Rat
      see all
    • 別名

      • ALPS4 antibody
      • AV095280 antibody
      • GTPase NRas antibody
      • HRAS1 antibody
      • N ras antibody
      • N ras protein part 4 antibody
      • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
      • NRAS antibody
      • NRAS1 antibody
      • NS6 antibody
      • OTTHUMP00000013879 antibody
      • OTTMUSP00000023521 antibody
      • RASN_HUMAN antibody
      • Transforming protein N Ras antibody
      • Transforming protein N-Ras antibody
      • v ras neuroblastoma RAS viral oncogene homolog antibody
      see all

    画像

    • Western blot - Anti-NRAS antibody (ab77392)
      Western blot - Anti-NRAS antibody (ab77392)
      Anti-NRAS antibody (ab77392) at 0.5 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate in RIPA buffer. at 35 µg

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.

       

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (13)

    ab77392 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab77392 は 13 報の論文で使用されています。

    • Yan Y  et al. NRAS expression is associated with prognosis and tumor immune microenvironment in lung adenocarcinoma. J Cancer Res Clin Oncol 148:565-575 (2022). PubMed: 34746975
    • Ma Y  et al. Neuronal miR-29a protects from obesity in adult mice. Mol Metab 61:101507 (2022). PubMed: 35490865
    • Nam HJ  et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051
    • Gopal P  et al. The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation. Clin Cancer Res 28:5343-5358 (2022). PubMed: 36222846
    • Bery N  et al. A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS. Nat Commun 11:3233 (2020). PubMed: 32591521
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-NRAS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    MDCK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 27 2022

    Western blot abreview for Anti-NRAS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (COS-1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    COS-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 27 2022

    Western blot abreview for Anti-NRAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (3611-RF cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    25 µg
    Specification
    3611-RF cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 08 2022

    Immunoprecipitation abreview for Anti-NRAS antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Total protein in input
    250 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    MEFs
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 17 2021

    Western blot abreview for Anti-NRAS antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    25 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 26 2021

    ELISA abreview for Anti-NRAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (NRAS protein)
    Specification
    NRAS protein
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Type
    Direct
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 19 2020

    Immunoprecipitation abreview for Anti-NRAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (PANC-1 cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    PANC-1 cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 26 2020

    Western blot abreview for Anti-NRAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PANC-1 cells)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    20 µg
    Specification
    PANC-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NRAS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    small intestine
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    投稿 Jun 22 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NRAS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    small intestine
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    投稿 Jun 20 2018

    1-10 of 11 Abreviews or Q&A

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