Anti-NR2F6/EAR-2 抗体 (ab65012)
Key features and details
- Rabbit polyclonal to NR2F6/EAR-2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NR2F6/EAR-2 antibody
NR2F6/EAR-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NR2F6/EAR-2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65012の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC). -
組織特異性
Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2063 Human
- Omim: 132880 Human
- SwissProt: P10588 Human
- Unigene: 466148 Human
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別名
- EAR 2 antibody
- EAR-2 antibody
- EAR2 antibody
see all
画像
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Western blot - Anti-NR2F6/EAR-2 antibody (ab65012)All lanes : Anti-NR2F6/EAR-2 antibody (ab65012) at 1/500 dilution
Lane 1 : HeLa cell extract at 10-30µg
Lane 2 : 293 cell extract at 10-30µg
Lane 3 : HUVEC cell extract at 10-30µg
Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-NR2F6/EAR-2 antibody (ab65012)ab65012 at 1/500 - 1/1000 dilution staining NR2F6/EAR-2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (ab65012)ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6/EAR-2 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab65012 は 2 報の論文で使用されています。
- Weatherford ET et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB . PubMed: 22278040
- Modica S et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human . PubMed: 19818784