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遺伝子ノックアウト細胞株 検証済

Anti-NQO1 抗体 (ab34173)

Reviews (9)Q&A (3)References (162)
Western blot - Anti-NQO1 antibody (ab34173)
  • Western blot - Anti-NQO1 antibody (ab34173)
  • Immunocytochemistry - Anti-NQO1 antibody (ab34173)
  • Western blot - Anti-NQO1 antibody (ab34173)

Key features and details

  • Rabbit polyclonal to NQO1
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-NQO1 antibody [EPR3309] (ab80588)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-NQO1 antibody
    NQO1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to NQO1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type HAP1 whole cell lysate. HepG2 and MCF7 whole cell lysate. Human Kidney Whole Tissue Lysate. Recombinant human NQO1 protein. ICC: HeLa cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34173の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 1 - 5 µg/ml.
WB (5)
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
特記事項
ICC
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能

    The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

  • 配列類似性

    Belongs to the NAD(P)H dehydrogenase (quinone) family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H quinone dehydrogenase 1 antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

画像

  • Western blot - Anti-NQO1 antibody (ab34173)
    Western blot - Anti-NQO1 antibody (ab34173)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: NQO1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab34173 observed at 31 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab34173 was shown to specifically react with NQO1 in wild-type HAP1 cells as signal was lost in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. ab34173 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NQO1 antibody (ab34173)
    Western blot - Anti-NQO1 antibody (ab34173)
    Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Immunocytochemistry - Anti-NQO1 antibody (ab34173)
    Immunocytochemistry - Anti-NQO1 antibody (ab34173)

    ab34173 staining NQO1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34173 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-NQO1 antibody (ab34173)
    Western blot - Anti-NQO1 antibody (ab34173)
    Anti-NQO1 antibody (ab34173) at 1 µg/ml + Recombinant human NQO1 protein (ab59663) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa


    Exposure time: 30 seconds

参考文献 (162)

ab34173 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab34173 は 162 報の論文で使用されています。

  • Koch M  et al. Quantitative Proteomics Identifies Reduced NRF2 Activity and Mitochondrial Dysfunction in Atopic Dermatitis. J Invest Dermatol 143:220-231.e7 (2023). PubMed: 36108803
  • Chen H  et al. Design and characterization of a heterobifunctional degrader of KEAP1. Redox Biol 59:102552 (2023). PubMed: 36473314
  • Yang Y  et al. Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage. Front Immunol 14:1089576 (2023). PubMed: 36761756
  • Zeng L  et al. Dexmedetomidine improves DM-induced oxidative stress injury to protect liver function through Nrf2 pathway. Am J Transl Res 15:1715-1729 (2023). PubMed: 37056865
  • Cho YJ  et al. Mild Oxidative Stress Induced by Sodium Arsenite Reduces Lipocalin-2 Expression Levels in Cortical Glial Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37958847
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 12 Abreviews or Q&A

Western blot abreview for Anti-NQO1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (lung)
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Loading amount
20 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 19 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NQO1 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
ovary
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 02 2015

Immunohistochemistry (Frozen sections) abreview for Anti-NQO1 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Permeabilization
Yes - it is an nuclear protein
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Dec 08 2011

Western blot abreview for Anti-NQO1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart, Skeletal muscle)
Loading amount
30 µg
Specification
Heart, Skeletal muscle
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 30°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Nov 30 2011

Western blot abreview for Anti-NQO1 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney (HK2))
Loading amount
10 µg
Specification
kidney (HK2)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 15 2011

Western blot abreview for Anti-NQO1 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
10 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 28 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NQO1 antibody

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas, Pancreatic ductal adenocarcinoma)
Specification
Pancreas, Pancreatic ductal adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 04 2009

Question



Based on the immunogen used to generate this antibody, do you agree that isoforms 2 and 3 of NQO1 would likely be picked up by this antibody if they were present in a sample?

Read More
Answer

Yes, based on the immunogen this antibody should be able to pick up all three isoforms on the NQO1 protein.



Read More

Question

Will this antibody work in dog?

Read More
Answer

Thank you for contacting us.

I couldn't find the dog sequence on Swiss Prot, so unfortunately I couldn't BLAST the sequences to compare them for you. The immunogen is from a.a. XXX - XXX so if you have access to the sequence, you may be able to do a comparison.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Dear xxxxx
NQO1 identification in frozen sections of rat brain.

We have tried ab34173 with rather poor and certainly unpublishable results. We wonder if ab2346 would be better, as it seems to work in mice. We have found one paper (attached) in which the authors claim to have used one of your anti-NQO1 antibodies and produced good images in rat brain frozen sections, but they failed to give the catalogue no. I would be very grateful if you were able to find some indication from your records which of the antibodies they used.

With kind regards

Read More
Answer

Thank you very much for supplying that information.

I am sorry to hear of your experience with ab34173. As requested, I am trying to track down which antibody was used in the paper you provided. However this is likely to be difficult as we supply our products through distributors in China (where the paper originates from) and I therefore also need their assistance to identify the order. I will however try my best. Unfortunately they haven't even mentioned which secondary antibody they used so I can'tevenreduce the number of possibilities by the species the antibody was raised in.

I have written an email to the author of the paper requesting the information so hopefully they will respond. I'll certainly keep you updated with whatever I find.

I am very sorry that you found ab34173 not to work as expected. I have looked into it and it seems we have had no similar reports and it is quite a popular antibody. It may unfortunately be that you received a faulty vial. Due to the nature of these products this does happen from time to time. If you would like I could provide you an alternative lot of this antibody to try free of charge?

Alternatively, if you would likeI could arrange for the other antibody, ab2346, which we discussed to be sent to you (again freeof charge)? Although as you say the reports where this has been used in frozen sections is primarily with mouse tissue, the antibody has been shown to detect the the rat protein and would be guaranteed by the Abpromise if you were not satisfied with the results. You would then be entitled to your money back or an alternative antibody if the problem is reported within 6 months of purchase.

In order to arrange for either of these options could you please provide me with the following information:

1. The order number (or approximate date of delivery and delivery address) of ab34173

2. A brief description of how the tissue was fixed?

3. What dilution of primary antibody was used and the incubation conditions?

4. What blockingconditions were used?

5. If you could provide an image of the results obtained that would be very helpful.

This information will also allow me to lookinto the problems encountered with ab34173 more fully and see if any additional testing needs to be implemented from our side.

I look forward to receiving your reply.

Read More

1-10 of 12 Abreviews or Q&A

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