Anti-NQO1 抗体 (ab2346)
Key features and details
- Goat polyclonal to NQO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NQO1 antibody
NQO1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NQO1 -
由来種
Goat -
特異性
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1) -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Rat, Human, Pig
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:C-SIPTDNQIKARK
(Peptide available asab22904) -
ポジティブ・コントロール
- WB: U251, HepG2, Human Kidney, Human Lung, Human Duodenum, Rat Kidney, Rat Lung and Rat Duodenum lysates. IHC-P: Human Kidney tissue. ICC/IF: HepG2 cells.
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特記事項
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-32 kDa.
Primary incubation for 1 hour at room temperature. |
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IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-32 kDa. Primary incubation for 1 hour at room temperature. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
配列類似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Rat Kidney lysate in RIPA buffer
Lane 2 : Rat Lung lysate in RIPA buffer
Lane 3 : Rat Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30-32 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Human Kidney lysate in RIPA buffer
Lane 2 : Human Lung lysate in RIPA buffer
Lane 3 : Human Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : U251 cell lysate in RIPA buffer
Lane 2 : HepG2 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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ab2346 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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ab2346 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (103)
ab2346 は 103 報の論文で使用されています。
- Hirose W et al. Selective Elimination of NRF2-Activated Cells by Competition With Neighboring Cells in the Esophageal Epithelium. Cell Mol Gastroenterol Hepatol 15:153-178 (2023). PubMed: 36115578
- Molina-Gonzalez I et al. Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration. Nat Commun 14:3372 (2023). PubMed: 37291151
- Chihara K et al. Macrophage specific restoration of the Nrf2 gene in whole-body knockout mice ameliorates steatohepatitis induced by lipopolysaccharide from Porphyromonas gingivalis through enhanced hepatic clearance. PLoS One 18:e0291880 (2023). PubMed: 37862331
- Liu Y et al. Nrf2 deficiency deteriorates diabetic kidney disease in Akita model mice. Redox Biol 58:102525 (2022). PubMed: 36335764
- Lee J et al. Lycopene Inhibits IL-6 Expression by Upregulating NQO1 and HO-1 via Activation of Nrf2 in Ethanol/Lipopolysaccharide-Stimulated Pancreatic Acinar Cells. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326169