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Anti-NPY1R 抗体 (ab35336)

Reviews (2)Q&A (1)References (4)
Western blot - Anti-NPY1R antibody (ab35336)

    Key features and details

    • Sheep polyclonal to NPY1R
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-NPY1R antibody
      NPY1R 一次抗体 製品一覧

    • 製品の詳細

      Sheep polyclonal to NPY1R

    • 由来種

      Sheep

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide: K-Q-A-S-P-V-A-F-K-K-I-N-N-N conjugated to KLH, corresponding to amino acids 365-378 of Human NPY1R

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab35336の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
    特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.

    ターゲット情報

    • 機能

      Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is NPY > [Pro-34] PYY, PYY and [Leu-31, Pro-34] NPY > NPY (2-36) > [Ile-31, Gln-34] PP and PYY (3-36) > PP > NPY free acid.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Neuropeptide Y receptor type 1 antibody
        • Neuropeptide Y receptor Y1 antibody
        • NPY1-R antibody
        • NPY1R antibody
        • NPY1R_HUMAN antibody
        • NPYR antibody
        • NPYY1 antibody
        see all

      画像

      • Western blot - Anti-NPY1R antibody (ab35336)
        Western blot - Anti-NPY1R antibody (ab35336)
        Anti-NPY1R antibody (ab35336) + Human Brain Lysate

        Predicted band size: 44 kDa

      参考文献 (4)

      ab35336 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab35336 は 4 報の論文で使用されています。

      • Dombrowski ME  et al. Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress. Neurospine 17:69-76 (2020). PubMed: 32252156
      • Yu W  et al. Inhibition of Y1 Receptor Promotes Osteogenesis in Bone Marrow Stromal Cells via cAMP/PKA/CREB Pathway. Front Endocrinol (Lausanne) 11:583105 (2020). PubMed: 33240219
      • Rice FL  et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962
      • Lundy FT  et al. Neuropeptide Y (NPY) and NPY Y1 receptor in periodontal health and disease. Arch Oral Biol 54:258-62 (2009). PubMed: 19010457

      レビューと Q&A

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      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-NPY1R antibody

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (rat skin (free floating 50 µm))
      Specification
      rat skin (free floating 50 µm)
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - Triton X-100, 0.2%
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jun 25 2012

      Western blot abreview for Anti-NPY1R antibody

       Good
      Abreviews
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (brain cortex)
      Loading amount
      100 µg
      Specification
      brain cortex
      Blocking step
      Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 8%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. paolo mele

      Verified customer

      投稿 Feb 13 2007

      Question

      IHC-Fr with rat frozen tissue
      fixation: PFA - pircric acide perfusion 30min
      post fix for 1 h
      Ab: 1/100-1/2000
      block: donkey serum
      2nd: Alexa 488, works well
      problem: no signal
      IHC-Fr untested
      suggested: try enzymatic antigen retrieval
      will send discount code for ab35336 trying in IHC-Fr

      Read More
      Answer

      Thank you for your phone call.
      I am sorry to hear that the antibody is not working in frozen sections, but unfortunately we have not tried this application and thus do not know for sure if the antibody would work or not in IHC-Fr.

      In order to help resolve this case, I'm happy to send youa testing discount code for having tried the antibody in an untested application.
      In addition, I might suggest you to try including an enzymatic antigen retrieval step as this might helpwith obtaining a signal.


      DISCOUNT CODE: xxx
      Expiration date: xxx

      I am very pleased to hear you would like to accept our offer and test ab35336 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com