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リコンビナントRabMAb

Anti-NPTXR + NPTX2 + NPTX1 抗体 [EPR25684-9] - BSA and Azide free (ab289870)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
  • Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25684-9] to NPTXR + NPTX2 + NPTX1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free

  • 製品の詳細

    Rabbit monoclonal [EPR25684-9] to NPTXR + NPTX2 + NPTX1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: IMR-32 whole cell lysate; Mouse brain tissue lysate; Rat brain tissue lysate; His-tagged human NPTXR recombinant protein fragment, His-tagged human NPTX1 full length recombinant protein (without signal peptide), recombinant human NPTX2 protein (without signal peptide). IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue. IHC-Fr: Mouse cerebrum (fresh) tissue; Rat cerebrum (fresh) tissue.

  • 特記事項

    ab289870 is a carrier-free version of ab289861. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289870の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30, 50 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30, 50 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse cerebrum (fresh). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    All lanes : Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (ab289861) at 1/1000 dilution

    Lane 1 : IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 3 : Mouse brain tissue lysate at 20 µg
    Lane 4 : Mouse skeletal muscle tissue lysate at 40 µg
    Lane 5 : Mouse spleen tissue lysate at 20 µg
    Lane 6 : Rat brain tissue lysate at 20 µg
    Lane 7 : Rat skeletal muscle tissue lysate at 40 µg
    Lane 8 : Rat spleen tissue lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBS. 

    Eposure times: 

    Lane1-2: 180 seconds;
    Lane3-8: 70 seconds. 

    Samples are non-boiled as boiling may cause protein aggregates.
    The observed MW are consistent with what has been described in the literature (PMID: 32847597, PMID: 32273328, PMID: 28440221)
    Low expression: Hela, skeletal muscle, spleen (PMID: 32847597, PMID: 32273328, PMID: 28440221)
    The bands nearby 40 kDa could be non-specific bands. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    All lanes : Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (ab289861) at 1/1000 dilution

    Lane 1 : His-tagged human NPTXR recombinant protein fragment 10ng
    Lane 2 : His-tagged human NPTX1 full length recombinant protein (without signal peptide) 10ng
    Lane 3 : Human NPTX2 full length recombinant protein (without signal peptide) 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBS. 

    Eposure times: 5.5 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Negative control (PMID: 9261167). No staining on mouse skeletal muscle (fresh). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab289861 for 30 mins at room temperature. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on rat cerebrum. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution. 

  • Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

    This data was developed using ab289861, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Negative control (PMID: 9261167). No staining on rat skeletal muscle (fresh). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution. 

  • Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)
    Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] - BSA and Azide free (ab289870)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289870 は論文での使用が確認できていません。

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