Anti-NPTX2 抗体 (ab69858)
Key features and details
- Rabbit polyclonal to NPTX2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NPTX2 antibody
NPTX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NPTX2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
A 16 amino acid peptide near the center of the human NPTX2
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69858の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
|
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner. -
組織特異性
Brain, pancreas, liver, heart and skeletal muscle. Highest levels are seen in the testis. -
配列類似性
Contains 1 pentaxin domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4885 Human
- Entrez Gene: 53324 Mouse
- Omim: 600750 Human
- SwissProt: P47972 Human
- SwissProt: O70340 Mouse
- Unigene: 3281 Human
- Unigene: 10099 Mouse
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別名
- apexin antibody
- NARP antibody
- neuronal activity-regulated pentaxin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody (ab69858)
Immunohistochemical analysis of paraffin-embedded human brain using ab69858 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-NPTX2 antibody (ab69858)All lanes : Anti-NPTX2 antibody (ab69858) at 1 µg/ml
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate with Blocking peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
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Western blot - Anti-NPTX2 antibody (ab69858)Lane 1 : Anti-NPTX2 antibody (ab69858) at 0.5 µg
Lane 2 : Anti-NPTX2 antibody (ab69858) at 1 µg/ml
All lanes : Mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDaDiluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-NPTX2 antibody (ab69858)Immunofluorescent analysis of 4% paraformaldehyde fixed human brain tissue labeling NPTX2 with ab69858 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab69858 は 9 報の論文で使用されています。
- Perna A et al. Revealing NOTCH-dependencies in synaptic targets associated with Alzheimer's disease. Mol Cell Neurosci 115:103657 (2021). PubMed: 34314836
- Lang Y et al. HOTAIR drives autophagy in midbrain dopaminergic neurons in the substantia nigra compacta in a mouse model of Parkinson's disease by elevating NPTX2 via miR-221-3p binding. Aging (Albany NY) 12:7660-7678 (2020). PubMed: 32396526
- Vilà González M et al. Endothelial Cells Derived From Patients With Diabetic Macular Edema Recapitulate Clinical Evaluations of Anti-VEGF Responsiveness Through the Neuronal Pentraxin 2 Pathway. Diabetes 69:2170-2185 (2020). PubMed: 32796081
- Xiang W et al. MicroRNA-96 is a potential tumor repressor by inhibiting NPTX2 in renal cell carcinoma. J Cell Biochem 121:1504-1513 (2020). PubMed: 31498486
- Xu C et al. NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/ß-catenin pathway via FZD6. Cell Death Dis 10:217 (2019). PubMed: 30833544
- Tang CZ et al. Overexpression of microRNA-301b accelerates hippocampal microglia activation and cognitive impairment in mice with depressive-like behavior through the NF-?B signaling pathway. Cell Death Dis 10:316 (2019). PubMed: 30962417
- Li T et al. Endometriosis alters brain electrophysiology, gene expression and increases pain sensitization, anxiety, and depression in female mice. Biol Reprod 99:349-359 (2018). PubMed: 29425272
- Lee FH et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Sci Rep 6:30458 (2016). AP ; Mouse . PubMed: 27461448
- Kone M et al. LKB1 and AMPK differentially regulate pancreatic ß-cell identity. FASEB J 28:4972-85 (2014). PubMed: 25070369