Anti-NPR-A 抗体 (ab14356)
Key features and details
- Rabbit polyclonal to NPR-A
- Suitable for: ICC, IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NPR-A antibody -
製品の詳細
Rabbit polyclonal to NPR-A -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
1/300.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/300. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. -
配列類似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation of the protein kinase-like domain is required for full activation by ANP. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4881 Human
- Entrez Gene: 24603 Rat
- Omim: 108960 Human
- SwissProt: P16066 Human
- SwissProt: P18910 Rat
- Unigene: 490330 Human
- Unigene: 10463 Rat
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別名
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
see all
画像
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ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab14356 は 19 報の論文で使用されています。
- Binder NK et al. Investigating the Effects of Atrial Natriuretic Peptide on the Maternal Endothelium to Determine Potential Implications for Preeclampsia. Int J Mol Sci 24:N/A (2023). PubMed: 37047162
- Forte M et al. NPPA/atrial natriuretic peptide is an extracellular modulator of autophagy in the heart. Autophagy 19:1087-1099 (2023). PubMed: 35998113
- Huang Y et al. Investigating the role of NPR1 in dilated cardiomyopathy and its potential as a therapeutic target for glucocorticoid therapy. Front Pharmacol 14:1290253 (2023). PubMed: 38026943
- Chang P et al. BNP protects against diabetic cardiomyopathy by promoting Opa1-mediated mitochondrial fusion via activating the PKG-STAT3 pathway. Redox Biol 62:102702 (2023). PubMed: 37116257
- Ang WF et al. Unveiling the potential role of natriuretic peptide receptor a isoforms in fine-tuning the cGMP production and tissue-specific function. Sci Rep 13:20439 (2023). PubMed: 37993528