Anti-NPHS2 抗体 (ab177242)
Key features and details
- Goat polyclonal to NPHS2
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-NPHS2 antibody
NPHS2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NPHS2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Pig
交差が予測される動物種: Cow
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免疫原
Synthetic peptide corresponding to Human NPHS2 aa 365-377 (C terminal) (Cysteine residue).
Sequence:SPSKPVEPLNPKK
Database link: NP_055440.1 -
ポジティブ･コントロール
- WB: Mouse, human and pig kidney lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab177242 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177242の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
組織特異性
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
関連疾患
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
配列類似性
Belongs to the band 7/mec-2 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 537633 Cow
- Entrez Gene: 7827 Human
- Entrez Gene: 170484 Mouse
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- SwissProt: Q91X05 Mouse
- Unigene: 412710 Human
- Unigene: 289099 Mouse
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別名
- nephrosis 2 antibody
- Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
- nephrosis 2, idiopathic, steroid resistant antibody
see all
画像
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Western blot - Anti-NPHS2 antibody (ab177242)All lanes : Anti-NPHS2 antibody (ab177242) at 0.3 µg/ml
Lane 1 : Human kidney lysate (in RIPA buffer)
Lane 2 : Mouse kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Western blot - Anti-NPHS2 antibody (ab177242)Anti-NPHS2 antibody (ab177242) at 1 µg/ml + Pig kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab177242 は論文での使用が確認できていません。
