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リコンビナントRabMAb

Anti-NP-I 抗体 [EPR25683-17] (ab300404)

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Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Anti-NP-I antibody [EPR25683-17] (AB300404)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25683-17] to NP-I
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NP-I antibody [EPR25683-17]
    NP-I 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25683-17] to NP-I

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, Hela transfected with siRNA specifically targeting NPTX1, Human hypothalamus tissue, IMR-32 (Human neuroblastoma neuroblast), His-tagged human NPTX1 recombinant protein. IP: Human hypothalamus tissue. ICC/IF: IMR-32 (human neuroblastoma neuroblast). Flow Cyt (intra): HeLa (human cervix adenocarcinoma epithelial cell).

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25683-17

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300404の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/400.
WB
1/1000. Predicted molecular weight: 47 kDa.
ICC/IF
1/50.
IP
1/30.
特記事項
Flow Cyt (Intra)
1/400.
WB
1/1000. Predicted molecular weight: 47 kDa.
ICC/IF
1/50.
IP
1/30.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    May mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. Can mediate the neuronal and glial uptake of the snake venom toxin taipoxin.

  • 配列類似性

    Contains 1 pentaxin domain.

  • 細胞内局在

    Cytoplasmic vesicle > secretory vesicle.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Neuronal pentraxin I antibody
      • Neuronal pentraxin-1 antibody
      • NP 1 antibody
      • NP-I antibody
      • NP1 antibody
      • Nptx1 antibody
      • NPTX1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      All lanes : Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

      Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : Hela transfected with siRNA specifically targeting NPTX1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 169 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      All lanes : Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

      Lane 1 : His-tagged human NPTX1 recombinant protein
      Lane 2 : His-tagged human NPTX2 recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with human NPTX2.

    • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      All lanes : Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

      Lane 1 : Human hypothalamus tissue lysate
      Lane 2 : Human heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: Human heart (PMID:7695898).

    • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution + IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This blot was developed using a higher sensitivity ECL substrate.

    • Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilised IMR-32 (human neuroblastoma neuroblast) labelling NP-I with ab300404 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasm staining in IMR-32 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain Microtubule at 1/200 dilution. The Nuclear counterstain was DAPI.

      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404)

      Flow cytometric analysis 4% paraformaldehyde fixed 90% methanol permealized HeLa (human cervix adenocarcinoma epithelial cell) with ab300404 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)(Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404)
      Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404)

      NP-I was immunoprecipitated from human hypothalamus tissue lysate 10 μg with ab300404 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300404 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/30 dilution was used as a secondary antibody. 

      Lane 1: Human hypothalamus tissue lysate 10 μg (Input)

      Lane 2: Human hypothalamus tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300404 in Human hypothalamus tissue lysate. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    • Anti-NP-I antibody [EPR25683-17] (AB300404)
      Anti-NP-I antibody [EPR25683-17] (AB300404)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300404 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300404 は論文での使用が確認できていません。

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