AB55831

Anti-NOX1 抗体

Anti-NOX1 antibody

(6 Reviews)

(58 Publications)

Rabbit Polyclonal NOX1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 58 publications. Immunogen corresponding to Synthetic Peptide within Human NOX1.

別名を表示する

MOX1, NOH1, NOX1, NADPH oxidase 1, NOX-1, Mitogenic oxidase 1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, MOX-1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-NOX1 antibody (AB55831)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NOX1 antibody (AB55831)

ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Synthetic Peptide within Human NOX1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5S8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified by antigen specific affinity chromatography.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NOX1 also known as NADPH oxidase 1 is an enzyme belonging to the NADPH oxidase family. It plays a mechanical role by generating reactive oxygen species (ROS) particularly superoxide from oxygen using NADPH as a substrate. NOX1 has a molecular weight of approximately 65 kDa. It is expressed abundantly in the colon and also found in vascular smooth muscle cells and the prostate. This enzyme shares a high degree of homology with other members of the NOX family which includes NOX2 and NOX4.

Biological function summary

NOX1 is involved in cellular processes like host defense cellular signaling and regulation of gene expression. It is part of a multi-protein complex that includes p22phox NOXA1 and NOXO1 which are essential for its enzyme activity. NOX1 contributes to maintaining normal cell function through controlled production of ROS which act as signaling molecules that modulate various physiological processes. In the colon NOX1 plays a role in mucosal defense and epithelial cell proliferation.

Pathways

NOX1 is intimately involved in the redox regulation and signaling pathways. It participates in the MAPK signaling pathway that influences cell proliferation and differentiation. NOX1 also plays a role in the PI3K/AKT pathway which is linked to cell survival and apoptosis. Proteins such as Rac1 and p47phox are known to interact with NOX1 in these pathways influencing downstream signaling events important for maintaining cell homeostasis.

NOX1 is associated with inflammatory diseases and cancer. Aberrant NOX1 activity contributes to chronic inflammatory conditions such as ulcerative colitis likely due to its role in ROS production leading to tissue damage. In the context of cancer particularly in colorectal cancer NOX1 overexpression correlates with enhanced tumor growth and survival likely through the activation of signaling pathways that promote cell proliferation. The interplay between NOX1 and proteins like NF-kB and COX-2 suggests its potential as a therapeutic target in these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.. Isoform NOH-1L. NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.

See full target information NOX1

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文献 (58)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:4279 PubMed38383562

2024

Kallistatin deficiency exacerbates neuronal damage after cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Hayoung Kim,Gil Joon Suh,Woon Yong Kwon,Kyung Su Kim,Yoon Sun Jung,Taegyun Kim,Heesu Park

Cell death discovery 9:461 PubMed38104087

2023

CB2 regulates oxidative stress and osteoclastogenesis through NOX1-dependent signaling pathway in titanium particle-induced osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaqiang Tao,Xueyan Li,Miao Chu,Qiufei Wang,Ping Li,Qibin Han,Kai Chen,Pengfei Zhu,Yuefeng Hao,Xing Yang,Dechun Geng,Ye Gu

International journal of molecular sciences 24: PubMed37445606

2023

Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

María Elena Soto,Israel Pérez-Torres,Linaloe Manzano-Pech,Elizabeth Soria-Castro,Almilcar Morales-Marín,Edgar Samuel Ramírez-Marroquín,Humberto Martínez-Hernández,Valentín Herrera-Alarcón,Verónica Guarner-Lans

Molecular biology reports 50:2067-2076 PubMed36542238

2022

Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh Kumar,Shubhankar Suman,Bo-Hyun Moon,Albert J Fornace,Kamal Datta

Frontiers in pharmacology 13:930614 PubMed36120348

2022

Ferroptosis induced by iron overload promotes fibrosis in ovarian endometriosis and is related to subpopulations of endometrial stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqin Zhang,Xinyu Liu,Mengqi Deng,Chunyu Xu,Yubo Zhang,Di Wu,Fan Tang,Ruiye Yang,Jinwei Miao

Cells 11: PubMed35954150

2022

Hyperoxaluria Induces Endothelial Dysfunction in Preglomerular Arteries: Involvement of Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Saenz-Medina,Mercedes Muñoz,Claudia Rodriguez,Cristina Contreras,Ana Sánchez,María José Coronado,Elvira Ramil,Martin Santos,Joaquín Carballido,Dolores Prieto

Phytomedicine : international journal of phytotherapy and phytopharmacology 103:154214 PubMed35689902

2022

Scutellarin acts on the AR-NOX axis to remediate oxidative stress injury in a mouse model of cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Minzhen Deng,Jingbo Sun,Lilin Peng,Yan Huang,Wen Jiang,Shuang Wu,Lihua Zhou,Sookja Kim Chung,Xiao Cheng

Oxidative medicine and cellular longevity 2022:4750671 PubMed35432717

2022

Epithelial Membrane Protein 1 Promotes Sensitivity to RSL3-Induced Ferroptosis and Intensifies Gefitinib Resistance in Head and Neck Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Liang Zhang,Changyu Yao,Yunxia Ma,Yehai Liu

Food & function 13:781-794 PubMed34981106

2022

Supplementation with cyanidin and delphinidin mitigates high fat diet-induced endotoxemia and associated liver inflammation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Cremonini,Dario E Iglesias,Karen E Matsukuma,Shelly N Hester,Steven M Wood,Mark Bartlett,Cesar G Fraga,Patricia I Oteiza

Aging 13:22588-22610 PubMed34584017

2021

GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhang,Wusan Wang,Maodi Xu,Haitang Xie,Zhichen Pu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-NOX1 抗体

Anti-NOX1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NOX1 antibody (AB55831)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (58)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise