Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-NOTCH4 抗体 [EPR18049] (ab184742)

Submit a review Submit a question References (16)
Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
  • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
  • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
  • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
  • Immunoprecipitation - Anti-NOTCH4 antibody [EPR18049] (ab184742)
  • Anti-NOTCH4 antibody [EPR18049] (ab184742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18049] to NOTCH4
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NOTCH4 antibody [EPR18049]
    NOTCH4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18049] to NOTCH4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human NOTCH4 fragment recombinant protein; Human fetal lung, placenta, fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, A549, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3 and F9 whole cell lysates; Mouse brain, heart, spleen and placenta lysates; Rat brain and heart lysates. IP: HeLa whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184742の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/60.
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 210 kDa).
特記事項
IP
1/60.
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 210 kDa).
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 機能

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.

  • 組織特異性

    Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

  • 配列類似性

    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 28 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.

  • 翻訳後修飾

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.

  • 細胞内局在

    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ16302 antibody
      • hNotch 4 antibody
      • hNotch4 antibody
      • INT3 antibody
      • Neurogenic locus notch homolog protein 4 antibody
      • NOTC4_HUMAN antibody
      • Notch 4 antibody
      • Notch 4 intracellular domain antibody
      • Notch homolog 4 antibody
      • Notch4 antibody
      • NOTCH4 protein antibody
      see all

    画像

    • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      All lanes : Anti-NOTCH4 antibody [EPR18049] (ab184742) at 1/2000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (Agarose) (ab97052) at 1/100000 dilution

      Predicted band size: 210 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,2 and 3: 15 seconds;Lane 4: 3 minute.

    • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      All lanes : Anti-NOTCH4 antibody [EPR18049] (ab184742) at 1/2000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human placenta lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Human fetal heart lysate
      Lane 5 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 210 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,3,4 and 5: 3 minutes; Lane 2: 30 seconds.

      The bands beneath the 150 kD NECD band are likely to be the degradation fragments.

    • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Anti-NOTCH4 antibody [EPR18049] (ab184742) at 1/1000 dilution + Human NOTCH4 fragment recombinant protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 210 kDa


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Western blot - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      All lanes : Anti-NOTCH4 antibody [EPR18049] (ab184742) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat brain lysate
      Lane 5 : Rat heart lysate
      Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 9 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 10 : Mouse placenta lysate
      Lane 11 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 210 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,2,3,4 and 5: 1 minute; Lane 6, 7, 8 and 9: 15 seconds; Lane 10 and 11: 30 seconds.

      The bands beneath the 150 kD NECD band are likely to be the degradation fragments.

    • Immunoprecipitation - Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Immunoprecipitation - Anti-NOTCH4 antibody [EPR18049] (ab184742)

      NOTCH4 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184742 at 1/60 dilution.

      Western blot was performed from the immunoprecipitate using ab184742 at 1/5000 dilution.

      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

      Lane 1: HeLa whole cell lysate, 10µg (Input).

      Lane 2: ab184742 IP in HeLa whole cell lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184742 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-NOTCH4 antibody [EPR18049] (ab184742)
      Anti-NOTCH4 antibody [EPR18049] (ab184742)

    参考文献 (16)

    ab184742 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184742 は 16 報の論文で使用されています。

    • Zhang Y  et al. Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway. Environ Toxicol 38:1628-1640 (2023). PubMed: 36988346
    • Sharma D  et al. IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy. Commun Biol 5:479 (2022). PubMed: 35589941
    • Gao Y  et al. Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4. Front Pharmacol 13:797805 (2022). PubMed: 35846998
    • Xu K  et al. The Angiotensin-Converting Enzyme Inhibitory State Promotes the Transformation of Non-Small Cell Lung Cancer Blood Supply Pattern Toward Vasculogenic Mimicry Formation. Front Oncol 11:663671 (2021). PubMed: 34221978
    • Zhan C  et al. Effect of the Notch4/Dll4 signaling pathway in early gestational intrauterine infection on lung development. Exp Ther Med 22:972 (2021). PubMed: 34335914
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab184742.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com