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リコンビナントRabMAb

Anti-NOTCH3 抗体 [EPR25183-326] - BSA and Azide free (Detector) (ab300706)

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Sandwich ELISA - Anti-NOTCH3 antibody [EPR25183-326] - BSA and Azide free (Detector) (ab300706)
  • Anti-NOTCH3 [EPR25183-326] - BSA and Azide free (Detector) (AB300706)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25183-326] to NOTCH3 - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-NOTCH3 antibody [EPR25183-326] - BSA and Azide free (Detector)
    NOTCH3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25183-326] to NOTCH3 - BSA and Azide free (Detector)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab300706 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human NOTCH3. The recommended pair for sandwich ELISA is:
         Capture: ab300705, human NOTCH3 Capture Antibody (unconjugated)
         Detector: ab300706, human NOTCH3 Detector Antibody (unconjugated)
    The reference range value is 62.5-4000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300706の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25183-332] to NOTCH3 - BSA and Azide free (Capture) (ab300705).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25183-332] to NOTCH3 - BSA and Azide free (Capture) (ab300705).

ターゲット情報

  • 機能

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

  • 組織特異性

    Ubiquitously expressed in fetal and adult tissues.

  • 関連疾患

    Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.

  • 配列類似性

    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 34 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.

  • 翻訳後修飾

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.

  • 細胞内局在

    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CADASIL antibody
      • CASIL antibody
      • NOTC3_HUMAN antibody
      • Notch 3 antibody
      • Notch 3 intracellular domain antibody
      • Notch homolog 3 antibody
      • Notch3 antibody
      see all

    画像

    • Sandwich ELISA - Anti-NOTCH3 antibody [EPR25183-326] - BSA and Azide free (Detector) (ab300706)
      Sandwich ELISA - Anti-NOTCH3 antibody [EPR25183-326] - BSA and Azide free (Detector) (ab300706)
      Sandwich ELISA of ab300704 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
    • Anti-NOTCH3 [EPR25183-326] - BSA and Azide free (Detector) (AB300706)
      Anti-NOTCH3 [EPR25183-326] - BSA and Azide free (Detector) (AB300706)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300706 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300706 は論文での使用が確認できていません。

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