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AB313453

Anti-Notch2 抗体 [RP23040135]

Anti-Notch2 antibody [RP23040135]

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Rabbit Recombinant Multiclonal Notch2 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NOTCH2.

別名を表示する

Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Notch2 Antibody [RP23040135] (AB313453)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Notch2 Antibody [RP23040135] (AB313453)

Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of Notch-2 using ab313453 (2 ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (1 : 2000). Panel a) shows representative cells that were stained for detection and localization of Notch-2 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1 : 300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic and nuclear localization of Notch-2. Panel e) represents control cells with no primary antibody to assess background.

Western blot - Anti-Notch2 Antibody [RP23040135] (AB313453)
  • WB

Supplier Data

Western blot - Anti-Notch2 Antibody [RP23040135] (AB313453)

All lanes:

Western blot - Anti-Notch2 Antibody [RP23040135] (ab313453) at 1 µg/mL

Lane 1:

Whole cell extracts of U87-MG at 30 µg

Lane 2:

Whole cell extracts of Karpas-299 at 30 µg

Lane 3:

Whole cell extracts of MCF-7 at 30 µg

Lane 4:

Whole cell extracts of HEK-293 at 30 µg

Lane 5:

Whole cell extracts of SH-SY5Y at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

true

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

RP23040135

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human NOTCH2.

Q04721

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組合せ製品

Primary Antibodies

AB71559

Anti-Hes1 antibody

Western blot - Anti-Hes1 antibody (AB71559)

  • 4
  • 26
Primary Antibodies

AB240636

Anti-Ctip2 antibody [EPR23120-25]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

  • 5
  • 1
Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size|Advanced Validation

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB23426

Anti-NOTCH3 antibody

BOND RX™ Validated

Western blot - Anti-NOTCH3 antibody (AB23426)

  • 4
  • 22
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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製品の詳細

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.
Biological function summary

Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.

Pathways

Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.

Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed : 21378985, PubMed : 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed : 29149593). Positively regulates self-renewal of liver cancer cells (PubMed : 25985737).
See full target information NOTCH2
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Abcam product promise

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