Anti-Notch2 抗体 [B9] (ab245699)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [B9] to Notch2
- Suitable for: ICC/IF
- Reacts with: Human
製品の概要
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製品名
Anti-Notch2 antibody [B9]
Notch2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [B9] to Notch2 -
由来種
Rabbit -
特異性
An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2 but not NRR1.
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アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment. NRR domain of mouse Notch 2.
Database link: O35516 -
ポジティブ・コントロール
- ICC/IF: HeLa cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
B9 -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). -
組織特異性
Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo. -
関連疾患
Alagille syndrome 2
Hajdu-Cheney syndrome -
配列類似性
Belongs to the NOTCH family.
Contains 6 ANK repeats.
Contains 35 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. -
細胞内局在
Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737). - Information by UniProt
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参照データベース
- Entrez Gene: 4853 Human
- Entrez Gene: 18129 Mouse
- Omim: 600275 Human
- SwissProt: Q04721 Human
- SwissProt: O35516 Mouse
- Unigene: 487360 Human
- Unigene: 485843 Mouse
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別名
- AGS2 antibody
- hN2 antibody
- Motch B antibody
see all
画像
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PFA fixed, 0.15% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Notch2 using ab245699 at 10 µg/ml, 1 hr (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245699 は論文での使用が確認できていません。