JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245699

Anti-Notch2 抗体 [B9]

Anti-Notch2 antibody [B9]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal Notch2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Notch2.

別名を表示する

Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (AB245699)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (AB245699)

PFA fixed, 0.15% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Notch2 using ab245699 at 10 μg/ml, 1 hr (green) followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

B9

アイソタイプ

IgG

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Recombinant Fragment Protein within Mouse Notch2. The exact immunogen used to generate this antibody is proprietary information.

O35516

特異性

An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2 but not NRR1.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.02% Proclin 300 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.
Biological function summary

Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.

Pathways

Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.

Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed : 21378985, PubMed : 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed : 29149593). Positively regulates self-renewal of liver cancer cells (PubMed : 25985737).
See full target information NOTCH2
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 20:491-502 PubMed38819062

2024

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Feng,Juanji Li,Tingting Liu,Shiqi Huang,Xiangliang Chen,Shen Liu,Junshan Zhou,Hongdong Zhao,Ye Hong

Oncology research 30:77-87 PubMed37305324

2023

IGF2BP3-induced activation of EIF5B contributes to progression of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Li,Qian Wang,Hongfeng Liang,Shisheng Chen,Haiwen Chen,Yaoyong Lu,Changfu Yang
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB8926

Anti-Notch2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB8926)

  • 4
  • 2
Primary Antibodies

AB245325

Anti-Notch2 antibody

Western blot - Anti-Notch2 antibody (AB245325)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB23426

Anti-NOTCH3 antibody

BOND RX™ Validated

Western blot - Anti-NOTCH3 antibody (AB23426)

  • 4
  • 22
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com