AB181996

Anti-NOL11 抗体 [EPR14074-30]

Anti-NOL11 antibody [EPR14074-30]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NOL11 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

L14, NOL11, Nucleolar protein 11

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunofluorescent analysis of -20°C acetone-fixed Hela cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain : Dapi (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain : Dapi (blue).

Supplier Data

Western blot - Anti-NOL11 antibody [EPR14074-30] (AB181996)

All lanes:

Western blot - Anti-NOL11 antibody [EPR14074-30] (ab181996) at 1/5000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 146 kDa,81 kDa,91 kDa

Observed band size: 120 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14074-30

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NOL11 also known as Nucleolar Protein 11 plays a mechanical role in ribosome biogenesis. This protein is 77 kDa in mass and shows expression largely in the nucleolus of the cell. It participates in the proper processing of the 47S pre-ribosomal RNA (rRNA) into the mature 18S rRNA an essential step in the formation of functional ribosomes. NOL11 interacts with elements of the small-subunit (SSU) processome indicating its involvement in early stages of ribosome assembly.

Biological function summary

NOL11 contributes to cellular proliferation by ensuring efficient ribosome synthesis. It acts as a component of the SSU processome complex which is a large ribonucleoprotein complex essential for the maturation of pre-rRNA. The protein NOL11 serves as a critical factor required for the synthesis of rRNA a process that directly affects the production of ribosomes and subsequently protein synthesis influencing cell growth and division.

Pathways

The protein functions within two major biological pathways: ribosome biogenesis and the cell cycle. It has a central role in ribosome biogenesis impacting the processing of pre-rRNA and connecting with other rRNA processing factors like Utp21. NOL11 also influences the cell cycle by indirectly affecting cell growth through its impact on ribosome production tying in with growth control mechanisms that involve proteins like p53.

NOL11 shows associations with conditions such as North American Indian childhood cirrhosis and Diamond-Blackfan anemia. These connections arise due to its pivotal function in ribosome biogenesis; any impairment can disrupt cellular protein synthesis leading to such disorders. NOL11 interacts with proteins involved in these conditions like those in the Fanconi anemia related pathways which further establishes its role in disease pathogenesis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ribosome biogenesis factor. May be required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b.

See full target information NOL11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e02838 PubMed40548932

2025

CD74 Blockade Disrupts Endothelial Migrasome Signaling to Prevent Inflammatory Macrophage Differentiation and Inhibit Atherosclerotic Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kangnan Zhang,Jiong Chen,Zhenhua Zhu,Hong Hu,Qinghui Zhang,Rongrong Jia,Na Wang,Shihao Xiang,Yong Zhou,Yuehong Wang,Ling Xu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOL11 抗体 [EPR14074-30]

Anti-NOL11 antibody [EPR14074-30]

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Immunocytochemistry/ Immunofluorescence - Anti-NOL11 antibody [EPR14074-30] (AB181996)

Western blot - Anti-NOL11 antibody [EPR14074-30] (AB181996)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise