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AB250388

Anti-NOL10 抗体 [EPR14073-9] - BSA and Azide free

Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free

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Rabbit Recombinant Monoclonal NOL10 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

別名を表示する

Nucleolar protein 10, NOL10

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free (AB250388)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free (AB250388)

This data was developed using ab181161, the same antibody clone in a different buffer formulation.

Immunocytochemical analysis of Hela cells fixed in acetone at -20°C, labeling NOL10 using ab181161 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) 1/200 dilution (green) and counterstained with DAPI (blue)

Western blot - Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free (AB250388)
  • WB

Supplier Data

Western blot - Anti-NOL10 antibody [EPR14073-9] - BSA and Azide free (AB250388)

This data was developed using ab181161, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NOL10 antibody [EPR14073-9] (<a href='/products/primary-antibodies/nol10-antibody-epr14073-9-ab181161'>ab181161</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Huvec cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 80 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14073-9

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
Primary Antibodies

AB252819

Anti-BOP1 antibody [6H11]

Recombinant|Trial Size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-BOP1 antibody [6H11] (AB252819)

  • 0
  • 0
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

ab250388 is the carrier-free version of ab181161.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NOL10 also known as nucleolar protein 10 functions primarily in the nucleolus of the cell playing roles in ribosome biogenesis. NOL10 weighs approximately 73 kDa and is present in different human tissues most expressed in spleen and bone marrow. This protein participates in important cellular processes by interacting with ribosomal RNA precursors during ribosome assembly. Its positioning in the nucleolus hints at a focus on ribosome production.
Biological function summary

NOL10 contributes to ribosomal RNA synthesis and processing. It associates with ribosomal RNA processing complexes ensuring mature ribosomal RNA formation. NOL10 works closely with other nucleolar proteins forming part of a larger pre-ribosomal assembly essential for protein synthesis. By doing this NOL10 indirectly supports translation and cell growth.

Pathways

NOL10 is involved in the ribosome biogenesis and RNA processing pathways. It connects with pathways such as the ribosome synthesis pathway interacting with proteins like fibrillarin to promote production of functional ribosomes. It ensures efficient processing within these pathways linking the initial steps of ribosome creation to later stages of protein synthesis.

NOL10's role in ribosome biogenesis links it to conditions like dyskeratosis congenita and certain cancers. Abnormal expression or mutations in NOL10 can result in disrupted ribosomal function contributing to disease progression. It has a connection with proteins such as dyskerin in dyskeratosis congenita highlighting its role in maintaining normal cellular and tissue function.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information NOL10
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Abcam product promise

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