Anti-Nogo Receptor 抗体 [EPR11159] (ab184556)
![Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)](https://www.abcam.co.jp/ps/products/184/ab184556/Images/ab184556-454324-anti-nogo-receptor-antibody-epr11159-western-blot-neuro-2a.jpg "Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11159] to Nogo Receptor
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nogo Receptor antibody [EPR11159]
Nogo Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11159] to Nogo Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- SH-SY5Y, 293 and HepG2 cell lysates. Neuro-2a and C6 whole cell lysates. Mouse brain and hippocampus tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11159 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184556の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 51 kDa).
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| IP |
1/50 - 1/70.
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| 特記事項 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 51 kDa). |
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IP
1/50 - 1/70. |
追加情報
Is unsuitable for ICC.
ターゲット情報
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関連性
Nogo Receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. It is also a receptor for Nogo A (RTN4), OMG and MAG. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 65078 Human
- Entrez Gene: 65079 Mouse
- Entrez Gene: 113912 Rat
- Omim: 605566 Human
- SwissProt: Q9BZR6 Human
- SwissProt: Q99PI8 Mouse
- SwissProt: Q99M75 Rat
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別名
- NgR antibody
- Nogo 66 receptor antibody
- Nogo receptor antibody
see all
画像
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Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/5000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hippocampus tissue lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa -
![Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)](https://www.abcam.co.jp/ps/products/184/ab184556/Images/ab184556-216784-anti-nogo-receptor-antibody-epr11159-western-blot.jpg)
Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/50000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)](https://www.abcam.co.jp/ps/products/184/ab184556/Images/ab184556-216785-anti-nogo-receptor-antibody-epr11159-western-blot.jpg)
Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/10000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Nogo Receptor antibody [EPR11159] (ab184556)](https://www.abcam.co.jp/ps/products/184/ab184556/Images/ab184556-216786-anti-nogo-receptor-antibody-epr11159-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nogo Receptor antibody [EPR11159] (ab184556)Western blot analysis of Nogo Receptor in HepG2 cell lysate immunoprecipitated with ab184556 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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![Anti-Nogo Receptor antibody [EPR11159] (ab184556)](https://www.abcam.co.jp/ps/products/184/ab184556/Images/ab184556-4-benefits-of-recombinant-antibodies.png)
Anti-Nogo Receptor antibody [EPR11159] (ab184556)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab184556 は 4 報の論文で使用されています。
- Rodriguez CM et al. Targeting RTN4/NoGo-Receptor reduces levels of ALS protein ataxin-2. Cell Rep 41:111505 (2022). PubMed: 36288715
- Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). WB ; Rat . PubMed: 33356694
- Zeng K et al. RNAi targeting Nogo Receptor enhanced survival and proliferation of murine retinal ganglion cells during N-methyl-D-aspartate-induced optic nerve crush. Oncotarget 8:65009-65021 (2017). PubMed: 29029408
- Guo X & Liu X Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats. Mol Med Rep 16:2030-2036 (2017). PubMed: 28656312