Anti-NOD1 抗体 (ab105338)
Key features and details
- Rabbit polyclonal to NOD1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-NOD1 antibody
NOD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NOD1 -
由来種
Rabbit -
特異性
ab105338 is predicted to not cross-react with other CARD family members. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human NOD1 (C terminal).
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ポジティブ･コントロール
- EL4 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa. |
ターゲット情報
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機能
Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. -
組織特異性
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. -
配列類似性
Contains 1 CARD domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
翻訳後修飾
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition. -
細胞内局在
Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 107607 Mouse
- SwissProt: Q8BHB0 Mouse
- Unigene: 28498 Mouse
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別名
- CARD 4 antibody
- CARD4 antibody
- Caspase recruitment domain 4 antibody
see all
画像
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Western blot - Anti-NOD1 antibody (ab105338)Anti-NOD1 antibody (ab105338) at 1 µg/ml + EL4 cell lysate at 15 µg
Secondary
Anti-rabbit secondary antibody
Predicted band size: 108 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab105338 は 4 報の論文で使用されています。
- Huang FC et al. Beneficial effect of probiotics on Pseudomonas aeruginosa-infected intestinal epithelial cells through inflammatory IL-8 and antimicrobial peptide human beta-defensin-2 modulation. Innate Immun 26:592-600 (2020). PubMed: 32988256
- Zhang C et al. Columbianadin Suppresses Lipopolysaccharide (LPS)-Induced Inflammation and Apoptosis through the NOD1 Pathway. Molecules 24:N/A (2019). PubMed: 30717343
- Layunta E et al. NOD1 downregulates intestinal serotonin transporter and interacts with other pattern recognition receptors. J Cell Physiol 233:4183-4193 (2018). PubMed: 29030987
- Bae J et al. Activation of pattern recognition receptors in brown adipocytes induces inflammation and suppresses uncoupling protein 1 expression and mitochondrial respiration. Am J Physiol Cell Physiol 306:C918-30 (2014). PubMed: 24627558